Devices from the iGEM 2016 interlab
iGEM_2016_interlab_collection Version 1 (Collection)
This is a collection of devices that were used in the 2016 iGEM interlab study

Test1
Test1_collection Version 1 (Collection)
Test1

BBa_K1740000
BBa_K1740000 Version 1 (Component)
Dronpa 145N ORF (E. coli optimized)

gb snap
gbsnap_collection Version 1 (Collection)
snapgene

Free Genes Feature Libraries
FreeGenesFeatureLibraries_collection Version 1 (Collection)
A collection of Free Genes feature libraries for use with Synthetic Biology Curation Tools (SYNBICT).

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.

Altered Sequence Activities
AlteredSequenceActivities_collection Version 1 (Collection)
A set of activities to point to ways in which sequences have been altered between source and upload.

BBa_K1428000
BBa_K1428000 Version 1 (Component)
T7 promoter-driven BMC structural genes with a His6-tagged component for purification

BBa_K1740000
BBa_K1740000 Version 1 (Component)
Dronpa 145N ORF (E. coli optimized)

iGEM to SBOL conversion
igem2sbol Version 1 (Activity)
Conversion of the iGEM parts registry to SBOL2.1

no attachment test
noattachmenttest_collection Version 1 (Collection)
no attachment but still returned as twin

merR_MHT_c3
merR_MHT_c3 Version 1 (Component)
merR_MHT_c3.

merR_MHT_c4
merR_MHT_c4 Version 1 (Component)
merR_MHT_c4.

BBF10K_003498_backbone
BBF10K_003498_backbone Version 1 (Component)
synthetic DNA

IBMc050
IBMc050 Version 1 (Component)
pSB1A3-BbsI-M86N-cat-Plux2-B32-M86C-SapI

IBMc052
IBMc052 Version 1 (Component)
pSB3T5(BsaI-)-PBAD(SapI-)-B30-mCherry*-T

IBMc065
IBMc065 Version 1 (Component)
pSB3T5(BsaI-)-PBAD-B30-mCherry*(1-159)-GAG-M86N-cat-Plux2-B32-M86C-S-mCherry*(160-236)-T

IBMc071
IBMc071 Version 1 (Component)
pSB3T5(BsaI-)-PBAD(SapI-)-B33-ECF20-T

IBMc078
IBMc078 Version 1 (Component)
pSB3T5(BsaI-)-PBAD(SapI-)-B30-mCherry*(1-27)-BsaI-BsaI-mCherry*(218-236)-T

IBMc090
IBMc090 Version 1 (Component)
pTHSSd_1V-WTR1R2-BbsI(G)-kanR-lacZalpha-SapI-WTR1R2

IBMc097
IBMc097 Version 1 (Component)
pSB1C3(BsmBI-)-BsaI-mCherry*(27-218)-BsaI

IBMc101
IBMc101 Version 1 (Component)
pSB3T5(empty)

IBMc120
IBMc120 Version 1 (Component)
pSB4A3-PECF20-B30-mScarlet-I-B15

IBMc131
IBMc131 Version 1 (Component)
pSB3T5(BsaI-)-PBAD(SapI-)-B30-mCherry*-H6-Ter

IBMc175
IBMc175 Version 1 (Component)
pSB3T5(BsaI-)-PBAD(SapI-)-B33-ECF20(1-101)-G-M86-S-ECF20(102-193)-T

IBMc176
IBMc176 Version 1 (Component)
pSB3T5(BsaI-)-PBAD(SapI-)-B33-ECF20(1-101)-G-M86(1-6)-BsaI-BsaI-M86(149-154)-S-ECF20(102-193)-T

IBMc180
IBMc180 Version 1 (Component)
pSB1A3-BbsI-10aa-acVHH-T-cat-PBAD(SapI)-RiboJ-BCD22-acVHH-10aa-SapI

IBMc181
IBMc181 Version 1 (Component)
pSB1C3(BsmBI-,BsaI+)-BsaI-M86(6-149)-BsaI

IBMc186
IBMc186 Version 1 (Component)
pSB4A3(empty)

IBMc187
IBMc187 Version 1 (Component)
pSB3T5(BsaI-)-PBAD(SapI-)-B33-ECF20(1-101)-T-cat-PBAD(SapI-)-RiboJ-BCD22-ECF20(102-193)-T

IBMc188
IBMc188 Version 1 (Component)
pSB3T5(BsaI-)-PBAD(SapI-)-B33-ECF20(1-101)-2aa-acVHH-T-cat-PBAD(SapI-)-RiboJ-BCD22-acVHH-2aa-ECF20(102-193)-T

IBMc189
IBMc189 Version 1 (Component)
pSB3T5(BsaI-)-PBAD(SapI-)-B33-ECF20(1-101)-5aa-acVHH-T-cat-PBAD(SapI-)-RiboJ-BCD22-acVHH-5aa-ECF20(102-193)-T

IBMc190
IBMc190 Version 1 (Component)
pSB3T5(BsaI-)-PBAD(SapI-)-B33-ECF20(1-101)-10aa-acVHH-T-cat-PBAD(SapI-)-RiboJ-BCD22-acVHH-10aa-ECF20(102-193)-T

IBMc191
IBMc191 Version 1 (Component)
pSB3T5(BsaI-)-PBAD(SapI-)-B33-ECF20(1-101)-15aa-acVHH-T-cat-PBAD(SapI-)-RiboJ-BCD22-acVHH-15aa-ECF20(102-193)-T

IBMc226
IBMc226 Version 1 (Component)
pSB3A5*(BsaI-)-PBAD(SapI-)-tet1-TetR-Ter

IBMc227
IBMc227 Version 1 (Component)
pSB3A5*(BsaI-)-PBAD(SapI-)-tet1-TetR(1-20)-BsaI-BsaI-TetR(188-207)-Ter

IBMc228
IBMc228 Version 1 (Component)
pSB1C3(BsmBI-,BsaI+)-BsaI-TetR(20-188)-BsaI

IBMc233
IBMc233 Version 1 (Component)
pSB1A3-BbsI-M86N-T-cat-PPhlF-RiboJ-B32-M86C-SapI

IBMc249
IBMc249 Version 1 (Component)
pSB3A5*(BsaI-)(empty)

IBMc252
IBMc252 Version 1 (Component)
pSEVA471(BsbI+)

IBMc259
IBMc259 Version 1 (Component)
pSEVA471-PTet*-RiboJ10-B64-mScarlet-I-Ter

IBMc307
IBMc307 Version 1 (Component)
pSB3T5(BsaI-)-PBAD(SapI-)-B30-mCherry*(1-192)-G-M86-S-mCherry*(193-236)-H6-T

IBMc308
IBMc308 Version 1 (Component)
pSB3T5(BsaI-)-PBAD(SapI-)-B30-mCherry*(1-192)-G-M86(1-6)-BsaI-BsaI-M86(149-154)-S-mCherry*(193-236)-H6-T

IBMc318
IBMc318 Version 1 (Component)
pSB1A3-BbsI-G-ER-LBD-S-SapI

IBMc322
IBMc322 Version 1 (Component)
pSB3T5(BsaI-)-PBAD(SapI-)-B30-mCherry*(1-192)-GCS-mCherry*(193-236)-H6-Ter