IBMc175

IBMc175Version 1

Component

Created by:Baojun Wang,Trevor Y. H. Ho

pSB3T5(BsaI-)-PBAD(SapI-)-B33-ECF20(1-101)-G-M86-S-ECF20(102-193)-T



    • Details

    Types
    circular

    DnaRegion

    Roles
    engineered_plasmid

    SequencesIBMc175_seq (Version 1)

    Description

    pSB3T5(BsaI-)-PBAD(SapI-)-B33-ECF20(1-101)-G-M86-S-ECF20(102-193)-T
    Expression plasmid for ECF20 inserted with cis-M86 intein. Activity reference in acVHH-assisted bisection mapping experiment

    Notes

    Creator: Trevor Y. H. Ho
    Principal Investigator: Baojun Wang
    BioSafety Level: Level 1
    Backbone: pSB3T5
    Origin of replication: p15A
    Selection Markers: Tetracycline


    Sequence AnnotationLocationComponent / Role(s)
    tetR promoter
    tetR
    VF2
    RFC10 Prefix (full)
    araC
    PBAD(SapI-)
    BBa_B0033
    ECF20 1-101
    M86N
    M86C
    ECF20 102-193
    ECK120033736
    RFC10 Suffix
    VR
    p15A origin of replication
    		9,97
    98,1291
    1371,1390
    1494,1515
    complement(1516,2394)
    2422,2705
    2714,2724
    2731,3033
    3037,3336
    3337,3498
    3502,3777
    3789,3841
    3842,3862
    complement(3957,3976)
    4423,5247
    		promoter
    CDS
    primer_binding_site
    biological_region
    CDS
    promoter
    ribosome_entry_site
    CDS
    CDS
    CDS
    CDS
    terminator
    biological_region
    primer_binding_site
    origin_of_replication

    synbiohub#topLevel
    IBMc175/1
     
    synbiohub#ownedBy
    user/forbin
     
    genbank#date
    18-NOV-2020
    genbank#molecule
    ds-DNA

    Attachments

    © 2018 Newcastle University, University of Utah, Battelle Memorial Institute, and collaborators
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