IBMc181

IBMc181Version 1

Component

Created by:Trevor Y. H. Ho,Baojun Wang

pSB1C3(BsmBI-,BsaI+)-BsaI-M86(6-149)-BsaI



Types
circular

DnaRegion

Roles
engineered_plasmid

SequencesIBMc181_seq (Version 1)

Description

pSB1C3(BsmBI-,BsaI+)-BsaI-M86(6-149)-BsaI
Staging plasmid for insertion on the M86 intein

Notes

Creator: Trevor Y. H. Ho
Principal Investigator: Baojun Wang
BioSafety Level: Level 1
Backbone: pSB1C3
Origin of replication: pMB1
Selection Markers: Chloramphenicol


Sequence AnnotationLocationComponent / Role(s)
VF2
RFC10 Prefix (full)
M86
RFC10 Suffix
VR
rep (pMB1)
cat (BsmBI-)

455,474
573,594
602,1033
1041,1061
complement(1195,1214)
complement(1301,1915)
complement(2201,2517)
complement(1,343)
primer_binding_site
biological_region
CDS
biological_region
primer_binding_site
origin_of_replication
CDS

synbiohub#topLevel
IBMc181/1
 
synbiohub#ownedBy
user/forbin
 
genbank#date
17-NOV-2020
genbank#molecule
ds-DNA