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IBMc176

IBMc176 Version 1

Component

Created by: Baojun Wang,Trevor Y. H. Ho

pSB3T5(BsaI-)-PBAD(SapI-)-B33-ECF20(1-101)-G-M86(1-6)-BsaI-BsaI-M86(149-154)-S-ECF20(102-193)-T

Details

Types	circular DnaRegion
Roles	engineered_plasmid
Sequences	IBMc176_seq (Version 1)

Description

pSB3T5(BsaI-)-PBAD(SapI-)-B33-ECF20(1-101)-G-M86(1-6)-BsaI-BsaI-M86(149-154)-S-ECF20(102-193)-T
Expression vector to generate ORF insertion library in acVHH-assisted bisection mapping experiment

Notes

Creator: Trevor Y. H. Ho
Principal Investigator: Baojun Wang
BioSafety Level: Level 1
Backbone: pSB3T5
Origin of replication: p15A
Selection Markers: Tetracycline

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
VF2 ECK120033736 RFC10 Suffix M86C RFC10 Prefix (full) spacing sequence BBa_B0033 VR M86N PBAD(SapI-) tetR p15A origin of replication ECF20 102-193 araC tetR promoter ECF20 1-101	1371,1390 3395,3447 3448,3468 3086,3104 1494,1515 3063,3078 2714,2724 complement(3563,3582) 3037,3055 2422,2705 98,1291 4029,4853 3108,3383 complement(1516,2394) 9,97 2731,3033	primer_binding_site terminator biological_region CDS biological_region biological_region ribosome_entry_site primer_binding_site CDS promoter CDS origin_of_replication CDS CDS promoter CDS

Other Properties

genbank#date	18-NOV-2020
genbank#molecule	ds-DNA
synbiohub#ownedBy	user/forbin
synbiohub#topLevel	IBMc176/1

Member of these Collections

Intein_assisted_Bisection_Mapping

Attachments

Sequence Visualization

© 2018 Newcastle University, University of Utah, Battelle Memorial Institute, and collaborators
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