IBMc226

IBMc226 Version 1

Component

Created by: Baojun Wang,Trevor Y. H. Ho

pSB3A5*(BsaI-)-PBAD(SapI-)-tet1-TetR-Ter



    • Details

    Types
    circular

    DnaRegion

    Roles
    engineered_plasmid

    Sequences IBMc226_seq (Version 1)

    Description

    pSB3A5*(BsaI-)-PBAD(SapI-)-tet1-TetR-Ter
    Expression plasmid for TetR

    Notes

    Creator: Trevor Y. H. Ho
    Principal Investigator: Baojun Wang
    BioSafety Level: Level 1
    Backbone: pSB3A5*
    Origin of replication: p15A
    Selection Markers: Ampicillin


    Sequence Annotation Location Component / Role(s)
    ampR (BsaI-)
    PBAD(SapI-)
    p15A origin of replication
    VR
    RFC10 Suffix
    ECK120033736
    araC
    VF2
    tet1
    RFC10 Prefix (full)
    TetR
    		3,863
    1994,2277
    3580,4404
    complement(3114,3133)
    2999,3019
    2946,2998
    complement(1088,1966)
    943,962
    2286,2313
    1066,1087
    2314,2934
    		CDS
    promoter
    origin_of_replication
    primer_binding_site
    biological_region
    terminator
    CDS
    primer_binding_site
    ribosome_entry_site
    biological_region
    CDS

    genbank#date
    09-OCT-2020
    genbank#molecule
    ds-DNA
    synbiohub#ownedBy
    user/forbin
     
    synbiohub#topLevel
    IBMc226/1
     

    Attachments

    © 2018 Newcastle University, University of Utah, Battelle Memorial Institute, and collaborators
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