BBa_K1428000

BBa_K1428000 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1428000
Generated By: http://sbh.portalspace.dev/public/ecoli/igem2sbol/1
Created by: Lai Hei Ming
Date created: 2014-10-07 11:00:00
Date modified: 2015-05-08 01:10:23

T7 promoter-driven BMC structural genes with a His6-tagged component for purification



    • Details

    Types
    DnaRegion

    Roles
    engineered_region

    Composite

    Sequences BBa_K1428000_sequence (Version 1)

    Description

    T7 promoter diving the expression of His6-tagged EutS, with subsequent Plac-driven EutM, EutN, EutL, EutK

    Notes

    Clone BBa_K311004 into pET28a via NheI and NdeI

    Source

    BBa_K311004, pET28a

    Sequence Annotation Location Component / Role(s)
    T7 RBS
    signal peptide
    fusion linker (TGS)
    		13,29
    37,93
    94,103
    		feature/rbs ribosome_entry_site
    feature/cds CDS
    feature/cds CDS

    igem#sampleStatus
    It's complicated
    igem#status
    Planning
     
    synbiohub#ownedBy
    myers
     
    synbiohub#ownedBy
    user/fullerton2
     
    synbiohub#topLevel
    BBa_K1428000/1
     

    Attachments

    TypeNameSize
    Imagesource_generated_BBa_K142.png20.1 KB
    vnd.openxmlformats-officedocument.spreadsheetml.sheettest.xlsx8.71 KB
    xmlsbol.rdf116.63 KB
    vnd.openxmlformats-officedocument.spreadsheetml.sheettest.xlsx8.23 KB

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