IBMc071

IBMc071Version 1

Component

Created by:Baojun Wang,Trevor Y. H. Ho

pSB3T5(BsaI-)-PBAD(SapI-)-B33-ECF20-T



Types
circular

DnaRegion

Roles
engineered_plasmid

SequencesIBMc071_seq (Version 1)

Description

pSB3T5(BsaI-)-PBAD(SapI-)-B33-ECF20-T
ECF20 expression plasmid. Activity reference in IBM experiment on ECF20

Notes

Creator: Trevor Y. H. Ho
Principal Investigator: Baojun Wang
BioSafety Level: Level 1
Backbone: pSB3T5
Origin of replication: p15A
Selection Markers: Tetracycline


Sequence AnnotationLocationComponent / Role(s)
tetR promoter
tetR
VF2
RFC10 Prefix (full)
araC
PBAD(SapI-)
BBa_B0033
ECF20
ECK120033736
RFC10 Suffix
VR
p15A origin of replication
9,97
98,1291
1371,1390
1494,1515
complement(1516,2394)
2422,2705
2714,2724
2731,3309
3321,3373
3374,3394
complement(3489,3508)
3955,4779
promoter
CDS
primer_binding_site
biological_region
CDS
promoter
ribosome_entry_site
CDS
terminator
biological_region
primer_binding_site
origin_of_replication
synbiohub#topLevel
IBMc071/1
 
synbiohub#ownedBy
user/forbin
 
genbank#date
18-NOV-2020
genbank#molecule
ds-DNA