IBMc227

IBMc227Version 1

Component

Created by:Baojun Wang,Trevor Y. H. Ho

pSB3A5*(BsaI-)-PBAD(SapI-)-tet1-TetR(1-20)-BsaI-BsaI-TetR(188-207)-Ter



    • Details

    Types
    circular

    DnaRegion

    Roles
    engineered_plasmid

    SequencesIBMc227_seq (Version 1)

    Description

    pSB3A5*(BsaI-)-PBAD(SapI-)-tet1-TetR(1-20)-BsaI-BsaI-TetR(188-207)-Ter
    Expression vector to generate ORF insertion library in IBM on TetR

    Notes

    Creator: Trevor Y. H. Ho
    Principal Investigator: Baojun Wang
    BioSafety Level: Level 1
    Backbone: pSB3A5*
    Origin of replication: p15A
    Selection Markers: Ampicillin


    Sequence AnnotationLocationComponent / Role(s)
    ampR (BsaI-)
    VF2
    RFC10 Prefix (full)
    araC
    PBAD(SapI-)
    tet1
    TetR(1-20)
    spacing sequence
    TetR(188-207)
    ECK120033736
    RFC10 Suffix
    VR
    p15A origin of replication
    		174,1034
    1114,1133
    1237,1258
    complement(1259,2137)
    2165,2448
    2457,2484
    2485,2544
    2552,2567
    2575,2634
    2646,2698
    2699,2719
    complement(2814,2833)
    3280,4104
    		CDS
    primer_binding_site
    biological_region
    CDS
    promoter
    ribosome_entry_site
    CDS
    biological_region
    CDS
    terminator
    biological_region
    primer_binding_site
    origin_of_replication

    synbiohub#topLevel
    IBMc227/1
     
    synbiohub#ownedBy
    user/forbin
     
    genbank#date
    18-NOV-2020
    genbank#molecule
    ds-DNA

    Attachments

    © 2018 Newcastle University, University of Utah, Battelle Memorial Institute, and collaborators
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