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IBMc372

IBMc372 Version 1

Component

Created by: Baojun Wang,Trevor Y. H. Ho

pSB3T5(BsaI-)-PBAD(SapI-)-B33-ECF20(1-101)-G-M86(1-91)-HIG-ER-LBD-S-M86(92-154)-S-ECF20(102-193)-T

Details

Types	circular DnaRegion
Roles	engineered_plasmid
Sequences	IBMc372_seq (Version 1)

Description

pSB3T5(BsaI-)-PBAD(SapI-)-B33-ECF20(1-101)-G-M86(1-91)-HIG-ER-LBD-S-M86(92-154)-S-ECF20(102-193)-T
Expression plasmid for ECF20 split at 101/102 inserted with M86 intein split at 91/92 by ER-LBD

Notes

Creator: Trevor Y. H. Ho
Principal Investigator: Baojun Wang
BioSafety Level: Level 1
Backbone: pSB3T5
Origin of replication: p15A
Selection Markers: Tetracycline

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
ECF20 102-193 M86(1-91) ECK120033736 ER-LBD RFC10 Suffix tetR p15A origin of replication araC RFC10 Prefix (full) PBAD(SapI-) VR M86(92-154) BBa_B0033 ECF20 1-101 tetR promoter VF2	4258,4533 3037,3309 4545,4597 3319,4062 4598,4618 98,1291 5179,6003 complement(1516,2394) 1494,1515 2422,2705 complement(4713,4732) 4066,4254 2714,2724 2731,3033 9,97 1371,1390	CDS CDS terminator CDS biological_region CDS origin_of_replication CDS biological_region promoter primer_binding_site CDS ribosome_entry_site CDS promoter primer_binding_site

Other Properties

genbank#date	18-NOV-2020
genbank#molecule	ds-DNA
synbiohub#ownedBy	user/forbin
synbiohub#topLevel	IBMc372/1

Member of these Collections

Intein_assisted_Bisection_Mapping

Attachments

Sequence Visualization

© 2018 Newcastle University, University of Utah, Battelle Memorial Institute, and collaborators
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