IBMc372 1 pSB3T5(BsaI-)-PBAD(SapI-)-B33-ECF20(1-101)-G-M86(1-91)-HIG-ER-LBD-S-M86(92-154)-S-ECF20(102-193)-T Creator: Trevor Y. H. Ho Principal Investigator: Baojun Wang BioSafety Level: Level 1 Backbone: pSB3T5 Origin of replication: p15A Selection Markers: Tetracycline pSB3T5(BsaI-)-PBAD(SapI-)-B33-ECF20(1-101)-G-M86(1-91)-HIG-ER-LBD-S-M86(92-154)-S-ECF20(102-193)-T Expression plasmid for ECF20 split at 101/102 inserted with M86 intein split at 91/92 by ER-LBD Baojun Wang Trevor Y. H. Ho 18-NOV-2020 ds-DNA annotation1 1 M86(1-91) #84b0dc #84b0dc M86(1-91) range 1 3037 3309 annotation13 1 tetR #9eafd2 #9eafd2 tetR range 1 98 1291 annotation2 1 RFC10 Prefix (full) #d6b295 #d6b295 RFC10 Prefix (full) range 1 1494 1515 annotation7 1 ECF20 1-101 #ffef86 #ffef86 ECF20 1-101 range 1 2731 3033 annotation14 1 p15A origin of replication #b4abac #b4abac p15A origin of replication range 1 5179 6003 annotation0 1 ECF20 102-193 #ffef86 #ffef86 ECF20 102-193 range 1 4258 4533 annotation4 1 VR #b4abac #b4abac VR range 1 4713 4732 annotation8 1 tetR promoter #f58a5e #f58a5e tetR promoter range 1 9 97 annotation5 1 M86(92-154) #84b0dc #84b0dc M86(92-154) range 1 4066 4254 annotation10 1 ECK120033736 #d59687 #d59687 ECK120033736 range 1 4545 4597 annotation15 1 araC #75c6a9 #75c6a9 araC range 1 1516 2394 annotation12 1 RFC10 Suffix #9eafd2 #9eafd2 RFC10 Suffix range 1 4598 4618 annotation9 1 VF2 #75c6a9 #75c6a9 VF2 range 1 1371 1390 annotation11 1 ER-LBD #ffef86 #ffef86 ER-LBD range 1 3319 4062 annotation6 1 BBa_B0033 #ff9ccd #ff9ccd BBa_B0033 range 1 2714 2724 annotation3 1 PBAD(SapI-) #faac61 #faac61 PBAD(SapI-) range 1 2422 2705 IBMc372_seq 1 tactagaggagattctcatgtttgacagcttatcatcgataagctttaatgcggtagtttatcacagttaaattgctaacgcagtcaggcaccgtgtatgaaatctaacaatgcgctcatcgtcattctcggcaccgtcaccctggacgctgtaggcataggcttggttatgccggtactgccgggcctcttgcgggatatcgtccattccgacagtattgccagtcactatggcgtgctgcttgcgctctatgcgttgatgcaatttctttgcgcacccgttctcggagccctgtccgaccgctttggccgccgtccagtcctgctcgcttcgctccttggagccactatcgactacgcgatcatggcgaccacacccgtcctgtggattctctacgccggacgcatcgtggcgggcatcacgggtgccacaggtgcggttgctggtgcctatatcgccgacatcaccgatggggaagatcgggctcgccacttcgggctcatgagcgcttgtttcggcgtgggtatggtggcaggccccgtggccgggggactgttgggtgccatctccttgcatgcaccattccttgcggcggcggtgctcaacggcctcaacctcctcctgggctgcttccttatgcaggaatcgcataagggagagcgccgtccgatgcccttgcgtgccttcaatccagtcagctccttccggtgggcgcggggcatgactatcgtcgccgcacttatgactgttttctttatcatgcaactcgtaggacaggttccggcagcgctctgggtcattttcggcgaggaccgctttcgctggagcgcgacgatgatcggcctgtcgcttgcggtattcggaatcttgcacgccctcgctcaagccttcgtcacgggccccgccaccaaacgtttcggcgagaagcaggccattatcgcgggcatggcggccgacgcgctgggctacgtcttgctggcgttcgcgacgcgcggctggatggccttccccattatgattcttctcgcttccggcggcatcggtatgcccgcgttgcaggccatgctgtcccgccaagtagatgacgaccatcagggacagcttcaagggtcgctcgcggctcttaccagcctcacttcgatcattggaccgctgatcgtcacggcgatttatgccgcctcggcgagcacatggaacgggttggcatggattgtaggtgccgccctttaccttgtctgcctccccgcgttgcgtcgcggtgcatggagccgggccacctcgacctaataatactagctccggcaaaaaaacgggcaaggtgtcaccaccctgccctttttctttaaaaccgaaaagattacttcgcgtttgccacctgacgtctaagaaaaggaatattcagcaatttgcccgtgccgaagaaaggcccacccgtgaaggtgagccagtgagttgattgctacgtaattagttagttagcccttagtgactcgaattcgcggccgcttctagagttatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcattttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtggtgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaaagatgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggtcgctgaaatgcggctggtgcgcttcatccgggcgaaagaaccccgtattggcaaatattgacggccagttaagccattcatgccagtaggcgcgcggacgaaagtaaacccactggtgataccattcgcgagcctccggatgacgaccgtagtgatgaatctctcctggcgggaacagcaaaatatcacccggtcggcaaacaaattctcgtccctgatttttcaccaccccctgaccgcgaatggtgagattgagaatataacctttcattcccagcggtcggtcgataaaaaaatcgagataaccgttggcctcaatcggcgttaaacccgccaccagatgggcattaaacgagtatcccggcagcaggggatcattttgcgcttcagccatacttttcatactcccgccattcagagaagaaaccaattgtccatattgcatcagacattgccgtcactgcgtcttttactggcActtctcgctaaccaaaccggtaaccccgcttattaaaagcattctgtaacaaagcgggaccaaagccatgacaaaaacgcgtaacaaaagtgtctataatcacggcagaaaagtccacattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccattactagagtcacacaggactactagatgaatgaaaccgatcctgatctggaactgctgaaacgtattggtaataatgatgcacaggccgttaaagaaatggttacccgtaaactgcctcgtctgctggcactggcaagtcgcctgctgggtgatgcagatgaagcacgtgatattgcacaagaaagttttctgcgcatttggaaacaggcagcaagctggcgtagcgaacaggcacgttttgatacctggctgcatcgtgttgcactgaatctgtgttatgatcgtctgcgtcgtcgtaaagaacatgtgccggttgatagcgaacatGGAtgcatctcgggagatagtttgatcagcttggcgagcacagggaaaagagttcctattaaggatttgttaggcgaaaaagattttgaaatatgggcaattaatgaacagacgatgaagctggaatcagctaaagttagtcgtgtattttgtaccggcaaaaagctagtctatactctaaaaactcgactaggtagaactatcaaggcaacagcaaatcatagatttttaactattgatggttggaaaagattagatgagctatctttaaaagagcatatTGGAAATAGCCTGGCACTGAGCCTGACCGCAGATCAGATGGTTAGCGCACTGCTGGATGCAGAACCGCCTATTCTGTATAGCGAATATGATCCGACACGTCCGTTTAGCGAAGCAAGCATGATGGGTCTGCTGACCAATCTGGCAGATCGTGAACTGGTTCATATGATTAATTGGGCAAAACGTGTTCCGGGTTTTGTTGATCTGACCCTGCATGATCAGGTTCATCTGCTGGAATGTGCATGGCTGGAAATTCTGATGATTGGTCTGGTTTGGCGTAGCATGGAACATCCGGGTAAACTGCTGTTTGCACCGAATCTGCTGCTGGATCGTAATCAGGGTAAATGTGTTGAAGGTATGGTGGAAATCTTTGATATGCTGCTGGCAACCAGCAGCCGTTTTCGTATGATGAATCTGCAAGGTGAAGAATTTGTTTGCCTGAAAAGCATTATTCTGCTGAACAGCGGTGTTTATACCTTTCTGAGCAGCACCCTGAAAAGTCTGGAAGAAAAAGATCATATCCATCGTGTGCTGGATAAAATCACCGATACACTGATTCATCTGATGGCAAAAGCCGGTCTGACCTTACAGCAGCAGCATCAGCGTCTGGCACAACTGCTGCTGATTCTGAGCCATATTCGTCATATGAGCAATAAAGGTATGGAACACCTGTACAGCATGAAATGCAAAAATGTTGTTCCGCTGTATGACCTGCTGTTAGAAATGCTGGACGCACATCGTCTGCATGCATCAcatattgctctaccccgtaaactagaaagctcctctttacaattggcaccagaaatagaaaagttgcctcagagtgatatttactgggaccccatcgtttctattacggagactggagtcgaagaggtttttgatttgactgtgccaggactacgtaactttgtcgccaatgacatcattgtacataacTCAgcctgtgaagcactggatacccgtccggcaccggatgaacagctggaagcaagcgcacagagccgtcgtatggcacaggcactggatcagctgccggatcgtcagcgtgaagcaattgttctgcagtattatcaagaactgagcaataccgaagcagcagcactgatgcaaattagcgttgaagccctggaaagcctgctgagccgtgcacgtcgtaatctgcgtagccatctggccgaagcaccgggtgcagatctgagcggtcgtcgcaaaccgtaatactagagaacgcatgagAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTtttattgcgctactagtagcggccgctgcaggagtcactaagggttagttagttagattagcagaaagtcaaaagcctccgaccggaggcttttgactaaaacttcccttggggttatcattggggctcactcaaaggcggtaatcagataaaaaaaatccttagctttcgctaaggatgatttctgctagagatggaatagactggatggaggcggataaagttgcaggaccacttctgcgctcggcccttccggctggctggtttattgctgataaatctggagccggtgagcgtggatctcgcggtatcattgcagcactggggccagatggtaagccctcccgtatcgtagttatctacacgacggggagtcaggcaactatggatgaacgaaatagacagatcgctgagataggtgcctcactgattaagcattggtaactgtcagaccaagtttactcatatatactttagattgatttaaaacttcatttttaatttaaaaggatctaggtgaagatcctttttgataatctcatgaccaaaatcccttaacgtgagttttcgttccactgagcgtcagaccccttaataagatgatcttcttgagatcgttttggtctgcgcgtaatctcttgctctgaaaacgaaaaaaccgccttgcagggcggtttttcgaaggttctctgagctaccaactctttgaaccgaggtaactggcttggaggagcgcagtcaccaaaacttgtcctttcagtttagccttaaccggcgcatgacttcaagactaactcctctaaatcaattaccagtggctgctgccagtggtgcttttgcatgtctttccgggttggactcaagacgatagttaccggataaggcgcagcggtcggactgaacggggggttcgtgcatacagtccagcttggagcgaactgcctacccggaactgagtgtcaggcgtggaatgagacaaacgcggccataacagcggaatgacaccggtaaaccgaaaggcaggaacaggagagcgcacgagggagccgccaggggaaacgcctggtatctttatagtcctgtcgggtttcgccaccactgatttgagcgtcagatttcgtgatgcttgtcaggggggcggagcctatggaaaaacggctttgccgcggccctctcacttccctgttaagtatcttcctggcatcttccaggaaatctccgccccgttcgtaagccatttccgctcgccgcagtcgaacgaccgagcgtagcgagtcagtgagcgaggaagcggaatatatcctgtatcacatattctgctgacgcaccggtgcagccttttttctcctgccacatgaagcacttcactgacaccctcatcagtgccaacatagtaagccagtatacactccgctagcgctgaggtctgcctcgtgaagaaggtgttgctgactcataccaggcctgaatcgccccatcatccagccagaaagtgagggagccacggttgatgagagctttgttgtaggtggaccagttggtgattttgaacttttgctttgccacggaacggtctgcgttgtcgggaagatgcgtgatctgatccttcaactcagcaaaagttcgatttattcaacaaagccacgttgtgtctcaaaatctctgatgttacattgcacaagataaaaatatatcatcatgaacaataaaactgtctgcttacataaacagtaatacaaggggtgtt