| Types | DnaRegion
|
| Roles | CDS
|
| Sequences | CnDatabase_Cas9Sequence (Version 1)
|
Description
This is the coding sequence of CRISPR associated protein 9 from the widely applied CRISPR-Cas system gene editing tool.
Notes
Originally, the Cas9 nucleotide sequence was PCR amplified from the Addgene plasmid #43802, and it has 2 SNPs in relation to the original one, but both sequences code the same aminoacid. At the C- terminal end of Cas9, there is the Nuclear Localization Signal (NLS), SV40 NLS.
References
DiCarlo, JE., Norville, JE., Mali, P., Rios, X., Aach, J., Church, GM., "Genome engineering in Saccharomyces cerevisiae using CRISPR-Cas systems.", Nucleic acids research, 2013, Apr 41(7): 4336-43, PMID:
23460208., DOI:
10.1093/nar/gkt135.
Arras, SD., Chua, SM., Wizrah, MS., Faint, JA., Yap, AS., Fraser, JA., "Targeted Genome Editing via CRISPR in the Pathogen Cryptococcus neoformans.", PloS one, 2016, 11(10): e0164322, PMID:
27711143., DOI:
10.1371/journal.pone.0164322.