Cryptococcus neoformans Database in Synthetic Biology Open Language

Description

This is a collection of Biological Genetic Parts of Cryptococcus neoformans containing 23 different promoters, coding sequences and terminators, which were previously used and whose efficacy has already been proven in this organism. This work was made in order to facilitate the build and assembly of genetic circuits and plasmids for the study of this pathogen.

Source

The parts were obtain through a extensive literature review and by search of plasmids in databases such as NCBI and AddGene.

References

McDade, HC., Cox, GM., "A new dominant selectable marker for use in Cryptococcus neoformans.", Medical mycology, 2001, Feb 39(1): 151-4, PMID: 11270405.

Luberto, C., Martinez-Mariño, B., Taraskiewicz, D., Bolaños, B., Chitano, P., Toffaletti, DL., Cox, GM., Perfect, JR., Hannun, YA., Balish, E., Del Poeta, M., "Identification of App1 as a regulator of phagocytosis and virulence of Cryptococcus neoformans.", The Journal of clinical investigation, 2003, Oct 112(7): 1080-94, PMID: 14523045.

Edman, JC., Kwon-Chung, KJ., "Isolation of the URA5 gene from Cryptococcus neoformans var. neoformans and its use as a selective marker for transformation.", Molecular and cellular biology, 1990, Sep 10(9): 4538-44, PMID: 2201894.

DiCarlo, JE., Norville, JE., Mali, P., Rios, X., Aach, J., Church, GM., "Genome engineering in Saccharomyces cerevisiae using CRISPR-Cas systems.", Nucleic acids research, 2013, Apr 41(7): 4336-43, PMID: 23460208., DOI: 10.1093/nar/gkt135.