IBMc471

IBMc471 Version 1

Component

Created by: Trevor Y. H. Ho,Baojun Wang

pSB1A3-PBAD(SapI-)-B30-mCherry*(1-173)-Ter



    • Details

    Types
    circular

    DnaRegion

    Roles
    engineered_plasmid

    Sequences IBMc471_seq (Version 1)

    Description

    pSB1A3-PBAD(SapI-)-B30-mCherry*(1-173)-Ter
    Expression plasmid for mCherry N-lobe. To proof mCherry split parts do not self-dimerize in mCherry BiFC experiment

    Notes

    Creator: Trevor Y. H. Ho
    Principal Investigator: Baojun Wang
    BioSafety Level: Level 1
    Backbone: pSB1A3
    Origin of replication: pMB1
    Selection Markers: Ampicllin


    Sequence Annotation Location Component / Role(s)
    VF2
    VR
    ampR
    PBAD(SapI-)
    mCherry*(1-173)
    L3S2P55
    rep (pMB1)
    BBa_B0030
    araC
    		982,1001
    complement(3000,3019)
    complement(1,861)
    2024,2307
    2337,2855
    2880,2936
    complement(3106,3720)
    2316,2330
    complement(1118,1996)
    		primer_binding_site
    primer_binding_site
    CDS
    promoter
    CDS
    terminator
    origin_of_replication
    ribosome_entry_site
    CDS

    genbank#date
    18-NOV-2020
    genbank#molecule
    ds-DNA
    synbiohub#ownedBy
    user/forbin
     
    synbiohub#topLevel
    IBMc471/1
     

    Attachments

    © 2018 Newcastle University, University of Utah, Battelle Memorial Institute, and collaborators
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