IBMc456

IBMc456Version 1

Component

Created by:Baojun Wang,Trevor Y. H. Ho

pSB3A5*(BsaI-)-PBAD(SapI-)-B30-H6-TetR(1-112)-5aa-SYNZIP17-BCD1-SYNZIP18-5aa-TetR(113-207)-HA-Ter



    • Details

    Types
    circular

    DnaRegion

    Roles
    engineered_plasmid

    SequencesIBMc456_seq (Version 1)

    Description

    pSB3A5*(BsaI-)-PBAD(SapI-)-B30-H6-TetR(1-112)-5aa-SYNZIP17-BCD1-SYNZIP18-5aa-TetR(113-207)-HA-Ter
    Expression plasmid for tagged TetR split at 112/113 with SYNZIP17 & SYNZIP18

    Notes

    Creator: Trevor Y. H. Ho
    Principal Investigator: Baojun Wang
    BioSafety Level: Level 1
    Backbone: pSB3A5*
    Origin of replication: p15A
    Selection Markers: Ampicillin


    Sequence AnnotationLocationComponent / Role(s)
    ampR (BsaI-)
    VF2
    RFC10 Prefix (full)
    araC
    PBAD(SapI-)
    BBa_B0030
    H6
    TetR(2-112)
    SYNZIP17
    BCD1
    RBS for CDS 1
    RBS for CDS2
    SYNZIP18
    TetR(113-207)
    HA tag
    ECK120033736
    RFC10 Suffix
    VR
    p15A origin of replication
    		3,863
    943,962
    1066,1087
    complement(1088,1966)
    1994,2277
    2286,2300
    2310,2327
    2328,2660
    2676,2801
    2805,2889
    2824,2831
    2875,2883
    2890,3015
    3031,3315
    3316,3342
    3354,3406
    3407,3427
    complement(3522,3541)
    3988,4812
    		CDS
    primer_binding_site
    biological_region
    CDS
    promoter
    ribosome_entry_site
    CDS
    CDS
    CDS
    biological_region
    ribosome_entry_site
    ribosome_entry_site
    CDS
    CDS
    CDS
    terminator
    biological_region
    primer_binding_site
    origin_of_replication

    synbiohub#topLevel
    IBMc456/1
     
    synbiohub#ownedBy
    user/forbin
     
    genbank#date
    18-NOV-2020
    genbank#molecule
    ds-DNA

    Attachments

    © 2018 Newcastle University, University of Utah, Battelle Memorial Institute, and collaborators
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