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IBMc454

IBMc454 Version 1

Component

Created by: Baojun Wang,Trevor Y. H. Ho

pSB3A5*(BsaI-)-PBAD(SapI-)-B30-H6-TetR-HA-Ter

Details

Types	circular DnaRegion
Roles	engineered_plasmid
Sequences	IBMc454_seq (Version 1)

Description

pSB3A5*(BsaI-)-PBAD(SapI-)-B30-H6-TetR-HA-Ter
Expression plasmid for tagged TetR

Notes

Creator: Trevor Y. H. Ho
Principal Investigator: Baojun Wang
BioSafety Level: Level 1
Backbone: pSB3A5*
Origin of replication: p15A
Selection Markers: Ampicillin

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
p15A origin of replication RFC10 Prefix (full) ECK120033736 RFC10 Suffix ampR (BsaI-) HA tag araC BBa_B0030 VR H6 TetR PBAD(SapI-) VF2	3618,4442 1066,1087 2984,3036 3037,3057 3,863 2946,2972 complement(1088,1966) 2286,2300 complement(3152,3171) 2310,2327 2328,2945 1994,2277 943,962	origin_of_replication biological_region terminator biological_region CDS CDS CDS ribosome_entry_site primer_binding_site CDS CDS promoter primer_binding_site

Other Properties

genbank#date	18-NOV-2020
genbank#molecule	ds-DNA
synbiohub#ownedBy	user/forbin
synbiohub#topLevel	IBMc454/1

Member of these Collections

Intein_assisted_Bisection_Mapping

Attachments

Sequence Visualization

© 2018 Newcastle University, University of Utah, Battelle Memorial Institute, and collaborators
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