IBMc454

IBMc454Version 1

Component

Created by:Baojun Wang,Trevor Y. H. Ho

pSB3A5*(BsaI-)-PBAD(SapI-)-B30-H6-TetR-HA-Ter



Types
circular

DnaRegion

Roles
engineered_plasmid

SequencesIBMc454_seq (Version 1)

Description

pSB3A5*(BsaI-)-PBAD(SapI-)-B30-H6-TetR-HA-Ter
Expression plasmid for tagged TetR

Notes

Creator: Trevor Y. H. Ho
Principal Investigator: Baojun Wang
BioSafety Level: Level 1
Backbone: pSB3A5*
Origin of replication: p15A
Selection Markers: Ampicillin


Sequence AnnotationLocationComponent / Role(s)
ampR (BsaI-)
VF2
RFC10 Prefix (full)
araC
PBAD(SapI-)
BBa_B0030
H6
TetR
HA tag
ECK120033736
RFC10 Suffix
VR
p15A origin of replication
3,863
943,962
1066,1087
complement(1088,1966)
1994,2277
2286,2300
2310,2327
2328,2945
2946,2972
2984,3036
3037,3057
complement(3152,3171)
3618,4442
CDS
primer_binding_site
biological_region
CDS
promoter
ribosome_entry_site
CDS
CDS
CDS
terminator
biological_region
primer_binding_site
origin_of_replication
synbiohub#topLevel
IBMc454/1
 
synbiohub#ownedBy
user/forbin
 
genbank#date
18-NOV-2020
genbank#molecule
ds-DNA