IBMc447

IBMc447Version 1

Component

Created by:Baojun Wang,Trevor Y. H. Ho

pSB3T5(BsaI-)-PPhlF-RiboJ-BCD1-SYNZIP18-5aa-mCherry*(122-236)-H6-Ter



    • Details

    Types
    circular

    DnaRegion

    Roles
    engineered_plasmid

    SequencesIBMc447_seq (Version 1)

    Description

    pSB3T5(BsaI-)-PPhlF-RiboJ-BCD1-SYNZIP18-5aa-mCherry*(122-236)-H6-Ter
    Expression plasmid for SYNZIP18-mCherry C-lobe. Used in mCherry BiFC experiment

    Notes

    Creator: Trevor Y. H. Ho
    Principal Investigator: Baojun Wang
    BioSafety Level: Level 1
    Backbone: pSB3T5
    Origin of replication: p15A
    Selection Markers: Tetracycline


    Sequence AnnotationLocationComponent / Role(s)
    tetR promoter
    tetR
    VF2
    RFC10 Prefix (full)
    L3S2P21
    PlacIQ
    phl2
    PhlF
    IOT
    L3S3P21
    PPhlF
    RiboJ
    BCD1
    RBS for CDS 1
    RBS for CDS2
    SYNZIP18
    mCherry*(122-236)
    H6
    ECK120033736
    RFC10 Suffix
    VR
    p15A origin of replication
    		9,97
    98,1291
    1371,1390
    1494,1515
    1516,1576
    1581,1634
    1635,1662
    1663,2265
    2275,2529
    2530,2582
    2602,2667
    2668,2742
    2746,2830
    2765,2772
    2816,2824
    2831,2956
    2972,3316
    3317,3334
    3346,3398
    3399,3419
    complement(3514,3533)
    3980,4804
    		promoter
    CDS
    primer_binding_site
    biological_region
    terminator
    promoter
    ribosome_entry_site
    CDS
    terminator
    terminator
    promoter
    insulator
    biological_region
    ribosome_entry_site
    ribosome_entry_site
    CDS
    CDS
    CDS
    terminator
    biological_region
    primer_binding_site
    origin_of_replication

    synbiohub#topLevel
    IBMc447/1
     
    synbiohub#ownedBy
    user/forbin
     
    genbank#date
    18-NOV-2020
    genbank#molecule
    ds-DNA

    Attachments

    © 2018 Newcastle University, University of Utah, Battelle Memorial Institute, and collaborators
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