IBMc417

IBMc417Version 1

Component

Created by:Baojun Wang,Trevor Y. H. Ho

pSB3T5(BsaI-)-PPhlF-RiboJ-B32-M86C-S-SrpR(53-213)-Ter



    • Details

    Types
    circular

    DnaRegion

    Roles
    engineered_plasmid

    SequencesIBMc417_seq (Version 1)

    Description

    pSB3T5(BsaI-)-PPhlF-RiboJ-B32-M86C-S-SrpR(53-213)-Ter
    M86C-SrpRC expression plasmid, to prove either lobe alone does not cause repression

    Notes

    Creator: Trevor Y. H. Ho
    Principal Investigator: Baojun Wang
    BioSafety Level: Level 1
    Backbone: pSB3T5
    Origin of replication: p15A
    Selection Markers: Tetracycline


    Sequence AnnotationLocationComponent / Role(s)
    tetR promoter
    tetR
    VF2
    RFC10 Prefix (full)
    cat (BsmBI-)
    cat promoter
    L3S2P21
    PlacIQ
    phl2
    PhlF
    IOT
    L3S3P21
    PPhlF
    RiboJ
    BBa_B0032
    M86C
    SrpR
    ECK120033736
    RFC10 Suffix
    VR
    p15A origin of replication
    		9,97
    98,1291
    1371,1390
    1494,1494
    complement(1517,2035)
    complement(2036,2133)
    2134,2194
    2199,2252
    2253,2280
    2281,2883
    2893,3147
    3148,3200
    3220,3285
    3286,3360
    3369,3381
    3391,3552
    3556,4038
    4050,4102
    4103,4123
    complement(4218,4237)
    4684,5508
    		promoter
    CDS
    primer_binding_site
    biological_region
    CDS
    promoter
    terminator
    promoter
    ribosome_entry_site
    CDS
    terminator
    terminator
    promoter
    insulator
    ribosome_entry_site
    CDS
    CDS
    terminator
    biological_region
    primer_binding_site
    origin_of_replication

    synbiohub#topLevel
    IBMc417/1
     
    synbiohub#ownedBy
    user/forbin
     
    genbank#date
    18-NOV-2020
    genbank#molecule
    ds-DNA

    Attachments

    © 2018 Newcastle University, University of Utah, Battelle Memorial Institute, and collaborators
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