Battelle SynBioHub

Created by:Trevor Y. H. Ho,Baojun Wang

pSB3T5(BsaI-)-PBAD(SapI-)-B30-mCherry(1-190)-PVG-M86N-cat-Plux2-B32-M86C-S-mCherry(191-236)-H6-T

Details

Types	circular DnaRegion
Roles	engineered_plasmid
Sequences	IBMc400_seq (Version 1)

Description

pSB3T5(BsaI-)-PBAD(SapI-)-B30-mCherry*(1-190)-PVG-M86N-cat-Plux2-B32-M86C-S-mCherry*(191-236)-H6-T
Expression plasmid for split mCherry construct derived from IBM, to prove protein splicing by M86 intein

Notes

Creator: Trevor Y. H. Ho
Principal Investigator: Baojun Wang
BioSafety Level: Level 1
Backbone: pSB3T5
Origin of replication: p15A
Selection Markers: Tetracycline/ Chloramphenicol

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
tetR promoter tetR VF2 RFC10 Prefix (full) araC PBAD(SapI-) BBa_B0030 mCherry* M86N ECK120029600 ECK120033737 cat (BsmBI-) cat promoter L3S2P55 luxR BBa_B0030 BBa_J23117 BBa_B0012 Plux2 BBa_B0032 M86C mCherry* H6 ECK120033736 RFC10 Suffix VR p15A origin of replication	9,97 98,1291 1371,1390 1494,1515 complement(1516,2394) 2422,2705 2714,2728 2735,3309 3314,3613 3625,3714 complement(3723,3779) complement(3808,4467) complement(4468,4565) complement(4574,4630) complement(4664,5419) complement(5426,5440) complement(5449,5483) 5484,5524 5533,5586 5595,5607 5617,5778 5782,5919 5920,5937 5949,6001 6002,6022 complement(6117,6136) 6583,7407	promoter CDS primer_binding_site biological_region CDS promoter ribosome_entry_site CDS CDS terminator terminator CDS promoter terminator CDS ribosome_entry_site promoter terminator promoter ribosome_entry_site CDS CDS CDS terminator biological_region primer_binding_site origin_of_replication

Other Properties

synbiohub#topLevel	IBMc400/1
synbiohub#ownedBy	user/forbin
genbank#date	18-NOV-2020
genbank#molecule	ds-DNA

Member of these Collections

Intein_assisted_Bisection_Mapping

IBMc400

IBMc400Version 1

Component