Battelle SynBioHub

Created by: Trevor Y. H. Ho,Baojun Wang

pSB3T5(BsaI-)-PBAD(SapI-)-B30-mCherry(1-187)-ANG-M86N-cat-Plux2-B32-M86C-S-mCherry(188-236)-H6-T

Details

Types	circular DnaRegion
Roles	engineered_plasmid
Sequences	IBMc398_seq (Version 1)

Description

pSB3T5(BsaI-)-PBAD(SapI-)-B30-mCherry*(1-187)-ANG-M86N-cat-Plux2-B32-M86C-S-mCherry*(188-236)-H6-T
Expression plasmid for split mCherry construct derived from IBM, to prove protein splicing by M86 intein

Notes

Creator: Trevor Y. H. Ho
Principal Investigator: Baojun Wang
BioSafety Level: Level 1
Backbone: pSB3T5
Origin of replication: p15A
Selection Markers: Tetracycline/ Chloramphenicol

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_B0032 M86N tetR promoter BBa_B0030 p15A origin of replication luxR RFC10 Suffix L3S2P55 ECK120033736 araC cat promoter H6 PBAD(SapI-) cat (BsmBI-) VF2 mCherry* ECK120033737 M86C BBa_J23117 ECK120029600 Plux2 mCherry* BBa_B0012 RFC10 Prefix (full) BBa_B0030 VR tetR	5586,5598 3305,3604 9,97 complement(5417,5431) 6583,7407 complement(4655,5410) 6002,6022 complement(4565,4621) 5949,6001 complement(1516,2394) complement(4459,4556) 5920,5937 2422,2705 complement(3799,4458) 1371,1390 5773,5919 complement(3714,3770) 5608,5769 complement(5440,5474) 3616,3705 5524,5577 2738,3295 5475,5515 1494,1515 2714,2728 complement(6117,6136) 98,1291	ribosome_entry_site CDS promoter ribosome_entry_site origin_of_replication CDS biological_region terminator terminator CDS promoter CDS promoter CDS primer_binding_site CDS terminator CDS promoter terminator promoter CDS terminator biological_region ribosome_entry_site primer_binding_site CDS

Other Properties

genbank#date	18-NOV-2020
genbank#molecule	ds-DNA
synbiohub#ownedBy	user/forbin
synbiohub#topLevel	IBMc398/1

Member of these Collections

Intein_assisted_Bisection_Mapping

IBMc398

IBMc398 Version 1

Component