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IBMc397

IBMc397Version 1

Component

Created by:Baojun Wang,Trevor Y. H. Ho

pSB3K3-TT-PBAD(SapI-)-B30-His-acVHH-15aa-T7RNAP(181+)-T

Details

Types	circular DnaRegion
Roles	engineered_plasmid
Sequences	IBMc397_seq (Version 1)

Description

pSB3K3-TT-PBAD(SapI-)-B30-His-acVHH-15aa-T7RNAP(181+)-T
Expression plasmid for split, evolved T7 RNA polymerase C-lobe fused to acVHH

Notes

Creator: Trevor Y. H. Ho
Principal Investigator: Baojun Wang
BioSafety Level: Level 1
Backbone: pSB3K3
Origin of replication: p15A
Selection Markers: Kanamycin

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
VF2 RFC10 Prefix (full) L3S3P21 L3S2P11 araC PBAD(SapI-) BBa_B0030 H6 acVHH 3x(GGGGS) linker T7 181+ BBa_B0015 RFC10 Suffix VR kanR p15A origin of replication	1133,1152 1251,1272 1273,1325 complement(1334,1390) complement(1399,2277) 2305,2588 2597,2611 2621,2638 2639,2995 2996,3040 3041,5155 5164,5292 5293,5313 complement(5447,5466) complement(5529,6344) complement(6676,6768) complement(1,732)	primer_binding_site biological_region terminator terminator CDS promoter ribosome_entry_site CDS CDS CDS CDS terminator biological_region primer_binding_site CDS origin_of_replication

Other Properties

synbiohub#topLevel	IBMc397/1
synbiohub#ownedBy	user/forbin
genbank#date	18-NOV-2020
genbank#molecule	ds-DNA

Member of these Collections

Intein_assisted_Bisection_Mapping

Attachments

Sequence Visualization

© 2018 Newcastle University, University of Utah, Battelle Memorial Institute, and collaborators
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