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IBMc395

IBMc395 Version 1

Component

Created by: Baojun Wang,Trevor Y. H. Ho

pSB3K3-TT-PBAD(SapI-)-B30-His-FKBP-14aa-T7RNAP(181+)-T

Details

Types	circular DnaRegion
Roles	engineered_plasmid
Sequences	IBMc395_seq (Version 1)

Description

pSB3K3-TT-PBAD(SapI-)-B30-His-FKBP-14aa-T7RNAP(181+)-T
Expression plasmid for split, evolved T7 RNA polymerase C-lobe fused to FKBP

Notes

Creator: Trevor Y. H. Ho
Principal Investigator: Baojun Wang
BioSafety Level: Level 1
Backbone: pSB3K3
Origin of replication: p15A
Selection Markers: Kanamycin

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_B0030 BBa_B0015 araC RFC10 Suffix L3S2P11 p15A origin of replication VR linker H6 L3S3P21 FKBP E coli optimized PBAD(SapI-) RFC10 Prefix (full) linker VF2 kanR T7 181+	3965,3979 6487,6615 complement(2767,3645) 6616,6636 complement(2702,2758) complement(1276,2100) complement(47,66) 4328,4345 3989,4006 2641,2693 4007,4327 3673,3956 2619,2640 4346,4363 2501,2520 complement(129,944) 4364,6478	ribosome_entry_site terminator CDS biological_region terminator origin_of_replication primer_binding_site CDS CDS terminator CDS promoter biological_region CDS primer_binding_site CDS CDS

Other Properties

genbank#date	18-NOV-2020
genbank#molecule	ds-DNA
synbiohub#ownedBy	user/forbin
synbiohub#topLevel	IBMc395/1

Member of these Collections

Intein_assisted_Bisection_Mapping

Attachments

Sequence Visualization

© 2018 Newcastle University, University of Utah, Battelle Memorial Institute, and collaborators
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