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IBMc370

IBMc370 Version 1

Component

Created by: Trevor Y. H. Ho,Baojun Wang

pSB3T5(BsaI-)-PBAD(SapI-)-B33-ECF20(1-101)-G-M86(1-78)-DGG-ER-LBD-S-M86(79-154)-S-ECF20(102-193)-T

Details

Types	circular DnaRegion
Roles	engineered_plasmid
Sequences	IBMc370_seq (Version 1)

Description

pSB3T5(BsaI-)-PBAD(SapI-)-B33-ECF20(1-101)-G-M86(1-78)-DGG-ER-LBD-S-M86(79-154)-S-ECF20(102-193)-T
Expression plasmid for ECF20 split at 101/102 inserted with M86 intein split at 78/79 by ER-LBD

Notes

Creator: Trevor Y. H. Ho
Principal Investigator: Baojun Wang
BioSafety Level: Level 1
Backbone: pSB3T5
Origin of replication: p15A
Selection Markers: Tetracycline

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
ECF20 1-101 PBAD(SapI-) ECF20 102-193 M86(1-78) ER-LBD VR M86(79-154) RFC10 Prefix (full) ECK120033736 VF2 RFC10 Suffix tetR tetR promoter araC p15A origin of replication BBa_B0033	2731,3033 2422,2705 4258,4533 3037,3270 3280,4023 complement(4713,4732) 4027,4254 1494,1515 4545,4597 1371,1390 4598,4618 98,1291 9,97 complement(1516,2394) 5179,6003 2714,2724	CDS promoter CDS CDS CDS primer_binding_site CDS biological_region terminator primer_binding_site biological_region CDS promoter CDS origin_of_replication ribosome_entry_site

Other Properties

genbank#date	18-NOV-2020
genbank#molecule	ds-DNA
synbiohub#ownedBy	user/forbin
synbiohub#topLevel	IBMc370/1

Member of these Collections

Intein_assisted_Bisection_Mapping

Attachments

Sequence Visualization

© 2018 Newcastle University, University of Utah, Battelle Memorial Institute, and collaborators
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