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IBMc337

IBMc337Version 1

Component

Created by:Baojun Wang,Trevor Y. H. Ho

pSB3T5(BsaI-)-PBAD(SapI-)-B30-mCherry(1-192)-G-deltaI-SMTR(WT)-S-mCherry(193-236)-H6-Ter

Details

Types	circular DnaRegion
Roles	engineered_plasmid
Sequences	IBMc337_seq (Version 1)

Description

pSB3T5(BsaI-)-PBAD(SapI-)-B30-mCherry*(1-192)-G-deltaI-SMTR(WT)-S-mCherry*(193-236)-H6-Ter
Expression plasmid for mCherry split at 192/193 inserted with Mtu RecA ?I-SMTR

Notes

Creator: Trevor Y. H. Ho
Principal Investigator: Baojun Wang
BioSafety Level: Level 1
Backbone: pSB3T5
Origin of replication: p15A
Selection Markers: Tetracycline

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
tetR promoter tetR VF2 RFC10 Prefix (full) araC PBAD(SapI-) BBa_B0030 mCherry(1-192) Mtu RecA 1-109 THRB MtuRecA 384-441 mCherry(193-236) H6 ECK120033736 RFC10 Suffix VR p15A origin of replication	9,97 98,1291 1371,1390 1494,1515 complement(1516,2394) 2422,2705 2714,2728 2735,3310 3314,3640 3647,4423 4430,4603 4607,4738 4739,4756 4768,4820 4821,4841 complement(4936,4955) 5402,6226	promoter CDS primer_binding_site biological_region CDS promoter ribosome_entry_site CDS CDS CDS CDS CDS CDS terminator biological_region primer_binding_site origin_of_replication

Other Properties

synbiohub#topLevel	IBMc337/1
synbiohub#ownedBy	user/forbin
genbank#date	18-NOV-2020
genbank#molecule	ds-DNA

Member of these Collections

Intein_assisted_Bisection_Mapping

Attachments

Sequence Visualization

© 2018 Newcastle University, University of Utah, Battelle Memorial Institute, and collaborators
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