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IBMc325

IBMc325 Version 1

Component

Created by: Trevor Y. H. Ho,Baojun Wang

pSB3T5(BsaI-)-PBAD(SapI-)-B30-mCherry(1-192)-G-37R3-2-S-mCherry(193-236)-H6-Ter

Details

Types	circular DnaRegion
Roles	engineered_plasmid
Sequences	IBMc325_seq (Version 1)

Description

pSB3T5(BsaI-)-PBAD(SapI-)-B30-mCherry*(1-192)-G-37R3-2-S-mCherry*(193-236)-H6-Ter
Expression plasmid for mCherry split at 192/193 inserted with Mtu RecA 37R3-2

Notes

Creator: Trevor Y. H. Ho
Principal Investigator: Baojun Wang
BioSafety Level: Level 1
Backbone: pSB3T5
Origin of replication: p15A
Selection Markers: Tetracycline

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
VR mCherry(193-236) RFC10 Prefix (full) H6 VF2 mCherry(1-192) PBAD(SapI-) Mtu RecA 37R3-2 BBa_B0030 tetR ECK120033736 araC RFC10 Suffix tetR promoter p15A origin of replication	complement(4885,4904) 4556,4687 1494,1515 4688,4705 1371,1390 2735,3310 2422,2705 3314,4552 2714,2728 98,1291 4717,4769 complement(1516,2394) 4770,4790 9,97 5351,6175	primer_binding_site CDS biological_region CDS primer_binding_site CDS promoter CDS ribosome_entry_site CDS terminator CDS biological_region promoter origin_of_replication

Other Properties

genbank#date	18-NOV-2020
genbank#molecule	ds-DNA
synbiohub#ownedBy	user/forbin
synbiohub#topLevel	IBMc325/1

Member of these Collections

Intein_assisted_Bisection_Mapping

Attachments

Sequence Visualization

© 2018 Newcastle University, University of Utah, Battelle Memorial Institute, and collaborators
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