merR_MHT_c4 1 merR_MHT_c4. 08-OCT-2024 SYN B0032_merR_MHT_r DNA synthetic DNA construct synthetic DNA construct Annotated with pLannotate v1.2.2 . . Exported Oct 8, 2024 from SnapGene 7.1.1 https://www.snapgene.com 1 (bases 1 to 4311) Direct Submission annotation6 1 oCMI235 oCMI235 color: #a020f0; direction: LEFT range 1 2101 2137 annotation4 1 rrnB T1 terminator rrnB T1 terminator pLannotate /other /note=color: #ffffff; direction: LEFT /identity/note=/match_length=100.0 /database/note=/fragment=False range 1 1592 1678 annotation9 1 oCMI234 oCMI234 color: #a020f0; direction: RIGHT range 1 2138 2160 annotation3 1 lacZ-alpha (fragment) 1 lacZ-alpha (fragment) /database/note=/fragment=True pLannotate /identity/note=/match_length=10.9 /other /note=color: #993366 TGRRFT range 1 1521 1539 annotation11 1 PmerR PmerR color: #ffffff range 1 2200 2241 annotation17 1 T7Te terminator T7Te terminator /other /note=color: #ffffff pLannotate /identity/note=/match_length=100.0 /database/note=/fragment=False range 1 3153 3180 annotation8 1 P479 P479 color: #ffffff range 1 2138 2178 annotation1 1 pBBR1 oriV pBBR1 oriV pLannotate /database/note=/fragment=False /identity/note=/match_length=100.3 /other /note=color: #ffff00 range 1 1 772 annotation16 1 rrnB T1 terminator rrnB T1 terminator /database/note=/fragment=False pLannotate /other /note=color: #ffffff /identity/note=/match_length=100.0 range 1 3066 3137 annotation10 1 lac UV5 promoter (fragment) lac UV5 promoter (fragment) pLannotate /identity/note=/match_length=83.9 /other /note=color: #ffffff; direction: LEFT /database/note=/fragment=True range 1 2143 2168 annotation7 1 B0032 B0032 color: #a6acb3 range 1 2125 2137 annotation0 1 synthetic DNA construct other DNA synthetic DNA construct range 1 1 4311 annotation14 1 RBS(B0034) RBS(B0034) color: #a6acb3 range 1 2317 2328 annotation12 1 RiboJ RiboJ color: #a6acb3 range 1 2242 2316 annotation13 1 sTRSV HHRz sTRSV HHRz /database/note=/fragment=False /identity/note=/match_length=96.2 pLannotate /other /note=color: #a6acb3; direction: RIGHT range 1 2244 2293 annotation2 1 pBBR1 Rep RIGHT pBBR1 Rep pLannotate /identity/note=/match_length=100.0 /database/note=/fragment=False /other /note=color: #993366 range 1 773 1435 annotation5 1 merR merR /identity/note=/match_length=100.0 /database/note=/fragment=False /other /note=color: #993366; direction: LEFT pLannotate range 1 1693 2124 annotation15 1 MHT MHT color: #993366; direction: RIGHT range 1 2329 3021 annotation18 1 KanR KanR /other /note=color: #993366; direction: LEFT pLannotate /identity/note=/match_length=100.0 /database/note=/fragment=False range 1 3250 4065 merR_MHT_c4_seq 1 gcggccaccggctggctcgcttcgctcggcccgtggacaaccctgctggacaagctgatggacaggctgcgcctgcccacgagcttgaccacagggattgcccaccggctacccagccttcgaccacatacccaccggctccaactgcgcggcctgcggccttgccccatcaatttttttaattttctctggggaaaagcctccggcctgcggcctgcgcgcttcgcttgccggttggacaccaagtggaaggcgggtcaaggctcgcgcagcgaccgcgcagcggcttggccttgacgcgcctggaacgacccaagcctatgcgagtgggggcagtcgaagggcgaagcccgcccgcctgccccccgagcctcacggcggcgagtgcgggggttccaagggggcagcgccaccttgggcaaggccgaaggccgcgcagtcgatcaacaagccccggaggggccactttttgccggagggggagccgcgccgaaggcgtgggggaaccccgcaggggtgcccttctttgggcaccaaagaactagatatagggcgaaatgcgaaagacttaaaaatcaacaacttaaaaaaggggggtacgcaacagctcattgcggcaccccccgcaatagctcattgcgtaggttaaagaaaatctgtaattgactgccacttttacgcaacgcataattgttgtcgcgctgccgaaaagttgcagctgattgcgcatggtgccgcaaccgtgcggcacccctaccgcatggagataagcatggccacgcagtccagagaaatcggcattcaagccaagaacaagcccggtcactgggtgcaaacggaacgcaaagcgcatgaggcgtgggccgggcttattgcgaggaaacccacggcggcaatgctgctgcatcacctcgtggcgcagatgggccaccagaacgccgtggtggtcagccagaagacactttccaagctcatcggacgttctttgcggacggtccaatacgcagtcaaggacttggtggccgagcgctggatctccgtcgtgaagctcaacggccccggcaccgtgtcggcctacgtggtcaatgaccgcgtggcgtggggccagccccgcgaccagttgcgcctgtcggtgttcagtgccgccgtggtggttgatcacgacgaccaggacgaatcgctgttggggcatggcgacctgcgccgcatcccgaccctgtatccgggcgagcagcaactaccgaccggccccggcgaggagccgcccagccagcccggcattccgggcatggaaccagacctgccagccttgaccgaaacggaggaatgggaacggcgcgggcagcagcgcctgccgatgcccgatgagccgtgttttctggacgatggcgagccgttggagccgccgacacgggtcacgctgccgcgccggtagcacttgggttgcgcagcaacccgtaagtgcgctgttccagactatcggctgtagccgcctcgccgccctataccttgtctggaacttgtaaaacgacggccagtcatgcataatccgcacgcatctggaataaggaagtgccattccgcctgacctatttgtcctactcaggagagcgttcaccgacaaacaacagataaaacgaaaggcccagtctttcgactgagcctttcgttttatttgggtctcacattttacggcatggcactaccggctaagcttgcaccaccctgcaggctggcaatcagcgggcagctcacattaccacgacgggcatggcaggcgcacaccagatcgctcagcacggcttccatgcgggccagatcggccatacgttcgcgcacgtccttcagtttatgttctgccaggctgctggcttcttcgcaatgggtaccatcctccagacgcagcagttcggcgatttcatccaggctaaagcccagacgctgggcgcttttcacaaaacgcacacgggtcacgtcggtttcaccatagcggcgaatgctaccatacggtttatccggttccggcagcaggcctttgcgctgatagaagcggatggtttccacgttcacgcctgcggtgcgtgcaaacacgccgatggtcaggttctccagattgttttccatctttcctgtgtgaccacacattatacgagccggaagcataaagtcttaactcgaaacgaattcaagcttgatatccgcttgactccgtacatgagtacggaagtaaggttacgctatagctgtcaccggatgtgctttccggtctgatgagtccgtgaggacgaaacagcctctacaaataattttgtttaaaaagaggagaaaatgtcgaccgtcgcaaatatcgcccccgtctttacgggtgattgcaaaaccattcccacgcccgaagaatgtgctaccttcttgtataaagtcgtgaactccggcggttgggagaagtgttgggtggaagaggtcattccatgggacctgggcgtgccgacccctttggtgttgcacttggtgaaaaacaatgccttgcctaatggcaaagggctcgtgccagggtgcgggggtgggtatgacgtggtcgcgatggcaaacccagaacggttcatggtggggttggacattagcgaaaacgctctgaagaaagcacgcgaaaccttttccacgatgccaaactccagctgtttcagcttcgtgaaggaagatgtgtttacgtggcgtccagagcaaccatttgacttcattttcgattacgtcttcttctgcgccattgaccctaaaatgcgccctgcctgggggaaagcgatgtatgaattgctgaaacctgatggggagctcatcacgctgatgtaccctattaccaaccacgagggcgggcctccattctcggtgtccgaatccgagtatgagaaggtgttggtcccgctgggtttcaaacagttgtcgctcgaggattactccgatttggctgtggagcctcggaaagggaaagagaagctggcccggtggaagaagatgaacaactaaaatggtgcttaaggatccaaactcgagtaaggatctccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgaagcggcgcgccgtcccgtcaagtcagcgtaatgctctgccagtgttacaaccaattaaccaattctgattagaaaaactcatcgagcatcaaatgaaactgcaatttattcatatcaggattatcaataccatatttttgaaaaagccgtttctgtaatgaaggagaaaactcaccgaggcagttccataggatggcaagatcctggtatcggtctgcgattccgactcgtccaacatcaatacaacctattaatttcccctcgtcaaaaataaggttatcaagtgagaaatcaccatgagtgacgactgaatccggtgagaatggcaaaagcttatgcatttctttccagacttgttcaacaggccagccattacgctcgtcatcaaaatcactcgcatcaaccaaaccgttattcattcgtgattgcgcctgagcgagacgaaatacgcgatcgctgttaaaaggacaattacaaacaggaatcgaatgcaaccggcgcaggaacactgccagcgcatcaacaatattttcacctgaatcaggatattcttctaatacctggaatgctgttttcccggggatcgcagtggtgagtaaccatgcatcatcaggagtacggataaaatgcttgatggtcggaagaggcataaattccgtcagccagtttagtctgaccatctcatctgtaacatcattggcaacgctacctttgccatgtttcagaaacaactctggcgcatcgggcttcccatacaatcgatagattgtcgcacctgattgcccgacattatcgcgagcccatttatacccatataaatcagcatccatgttggaatttaatcgcggcctcgagcaagacgtttcccgttgaatatggctcataacaccccttgtattactgtttatgtaagcagacagttttattgttcatgatgatatatttttatcttgtgcaatgtaacatcagagattttgagacacaacgtggctttccaggtggccggcccttcgcaaagtcgtgaccgcctacggcggctgcggcgccctacgggcttgctctccgggcttcgccctgcgcggtcgctgcgctcccttgccagcccgtggatatgtggacgatggccgcga