LOCUS B0032_merR_MHT_r 4311 bp DNA circular SYN 08-OCT-2024 DEFINITION merR_MHT_c4. ACCESSION merR_MHT_c4 VERSION merR_MHT_c4.1 KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 4311) AUTHORS . TITLE Direct Submission JOURNAL Exported Oct 8, 2024 from SnapGene 7.1.1 https://www.snapgene.com COMMENT Annotated with pLannotate v1.2.2 FEATURES Location/Qualifiers source 1..4311 /mol_type="other DNA" /organism="synthetic DNA construct" rep_origin 1..772 /label="pBBR1 oriV" /note="pLannotate" /note="/database/note=/fragment=False" /note="/identity/note=/match_length=100.3" /note="/other /note=color: #ffff00" rep_origin 773..1435 /direction="RIGHT" /label="pBBR1 Rep" /note="pLannotate" /note="/identity/note=/match_length=100.0" /note="/database/note=/fragment=False" /note="/other /note=color: #993366" CDS complement(1521..1539) /codon_start=1 /label="lacZ-alpha (fragment)" /note="/database/note=/fragment=True" /note="pLannotate" /note="/identity/note=/match_length=10.9" /note="/other /note=color: #993366" /translation="TGRRFT" terminator complement(1592..1678) /label="rrnB T1 terminator" /note="pLannotate" /note="/other /note=color: #ffffff; direction: LEFT" /note="/identity/note=/match_length=100.0" /note="/database/note=/fragment=False" gene complement(1693..2124) /label="merR" /note="/identity/note=/match_length=100.0" /note="/database/note=/fragment=False" /note="/other /note=color: #993366; direction: LEFT" /note="pLannotate" primer_bind complement(2101..2137) /label="oCMI235" /note="color: #a020f0; direction: LEFT" RBS 2125..2137 /label="B0032" /note="color: #a6acb3" promoter 2138..2178 /label="P479" /note="color: #ffffff" primer_bind 2138..2160 /label="oCMI234" /note="color: #a020f0; direction: RIGHT" promoter complement(2143..2168) /label="lac UV5 promoter (fragment)" /note="pLannotate" /note="/identity/note=/match_length=83.9" /note="/other /note=color: #ffffff; direction: LEFT" /note="/database/note=/fragment=True" promoter 2200..2241 /label="PmerR" /note="color: #ffffff" misc_feature 2242..2316 /label="RiboJ" /note="color: #a6acb3" misc_RNA 2244..2293 /label="sTRSV HHRz" /note="/database/note=/fragment=False" /note="/identity/note=/match_length=96.2" /note="pLannotate" /note="/other /note=color: #a6acb3; direction: RIGHT" misc_feature 2317..2328 /label="RBS(B0034)" /note="color: #a6acb3" gene 2329..3021 /label="MHT" /note="color: #993366; direction: RIGHT" terminator 3066..3137 /label="rrnB T1 terminator" /note="/database/note=/fragment=False" /note="pLannotate" /note="/other /note=color: #ffffff" /note="/identity/note=/match_length=100.0" terminator 3153..3180 /label="T7Te terminator" /note="/other /note=color: #ffffff" /note="pLannotate" /note="/identity/note=/match_length=100.0" /note="/database/note=/fragment=False" gene complement(3250..4065) /label="KanR" /note="/other /note=color: #993366; direction: LEFT" /note="pLannotate" /note="/identity/note=/match_length=100.0" /note="/database/note=/fragment=False" ORIGIN 1 gcggccaccg gctggctcgc ttcgctcggc ccgtggacaa ccctgctgga caagctgatg 61 gacaggctgc gcctgcccac gagcttgacc acagggattg cccaccggct acccagcctt 121 cgaccacata cccaccggct ccaactgcgc ggcctgcggc cttgccccat caattttttt 181 aattttctct ggggaaaagc ctccggcctg cggcctgcgc gcttcgcttg ccggttggac 241 accaagtgga aggcgggtca aggctcgcgc agcgaccgcg cagcggcttg gccttgacgc 301 gcctggaacg acccaagcct atgcgagtgg gggcagtcga agggcgaagc ccgcccgcct 361 gccccccgag cctcacggcg gcgagtgcgg gggttccaag ggggcagcgc caccttgggc 421 aaggccgaag gccgcgcagt cgatcaacaa gccccggagg ggccactttt tgccggaggg 481 ggagccgcgc cgaaggcgtg ggggaacccc gcaggggtgc ccttctttgg gcaccaaaga 541 actagatata gggcgaaatg cgaaagactt aaaaatcaac aacttaaaaa aggggggtac 601 gcaacagctc attgcggcac cccccgcaat agctcattgc gtaggttaaa gaaaatctgt 661 aattgactgc cacttttacg caacgcataa ttgttgtcgc gctgccgaaa agttgcagct 721 gattgcgcat ggtgccgcaa ccgtgcggca cccctaccgc atggagataa gcatggccac 781 gcagtccaga gaaatcggca ttcaagccaa gaacaagccc ggtcactggg tgcaaacgga 841 acgcaaagcg catgaggcgt gggccgggct tattgcgagg aaacccacgg cggcaatgct 901 gctgcatcac ctcgtggcgc agatgggcca ccagaacgcc gtggtggtca gccagaagac 961 actttccaag ctcatcggac gttctttgcg gacggtccaa tacgcagtca aggacttggt 1021 ggccgagcgc tggatctccg tcgtgaagct caacggcccc ggcaccgtgt cggcctacgt 1081 ggtcaatgac cgcgtggcgt ggggccagcc ccgcgaccag ttgcgcctgt cggtgttcag 1141 tgccgccgtg gtggttgatc acgacgacca ggacgaatcg ctgttggggc atggcgacct 1201 gcgccgcatc ccgaccctgt atccgggcga gcagcaacta ccgaccggcc ccggcgagga 1261 gccgcccagc cagcccggca ttccgggcat ggaaccagac ctgccagcct tgaccgaaac 1321 ggaggaatgg gaacggcgcg ggcagcagcg cctgccgatg cccgatgagc cgtgttttct 1381 ggacgatggc gagccgttgg agccgccgac acgggtcacg ctgccgcgcc ggtagcactt 1441 gggttgcgca gcaacccgta agtgcgctgt tccagactat cggctgtagc cgcctcgccg 1501 ccctatacct tgtctggaac ttgtaaaacg acggccagtc atgcataatc cgcacgcatc 1561 tggaataagg aagtgccatt ccgcctgacc tatttgtcct actcaggaga gcgttcaccg 1621 acaaacaaca gataaaacga aaggcccagt ctttcgactg agcctttcgt tttatttggg 1681 tctcacattt tacggcatgg cactaccggc taagcttgca ccaccctgca ggctggcaat 1741 cagcgggcag ctcacattac cacgacgggc atggcaggcg cacaccagat cgctcagcac 1801 ggcttccatg cgggccagat cggccatacg ttcgcgcacg tccttcagtt tatgttctgc 1861 caggctgctg gcttcttcgc aatgggtacc atcctccaga cgcagcagtt cggcgatttc 1921 atccaggcta aagcccagac gctgggcgct tttcacaaaa cgcacacggg tcacgtcggt 1981 ttcaccatag cggcgaatgc taccatacgg tttatccggt tccggcagca ggcctttgcg 2041 ctgatagaag cggatggttt ccacgttcac gcctgcggtg cgtgcaaaca cgccgatggt 2101 caggttctcc agattgtttt ccatctttcc tgtgtgacca cacattatac gagccggaag 2161 cataaagtct taactcgaaa cgaattcaag cttgatatcc gcttgactcc gtacatgagt 2221 acggaagtaa ggttacgcta tagctgtcac cggatgtgct ttccggtctg atgagtccgt 2281 gaggacgaaa cagcctctac aaataatttt gtttaaaaag aggagaaaat gtcgaccgtc 2341 gcaaatatcg cccccgtctt tacgggtgat tgcaaaacca ttcccacgcc cgaagaatgt 2401 gctaccttct tgtataaagt cgtgaactcc ggcggttggg agaagtgttg ggtggaagag 2461 gtcattccat gggacctggg cgtgccgacc cctttggtgt tgcacttggt gaaaaacaat 2521 gccttgccta atggcaaagg gctcgtgcca gggtgcgggg gtgggtatga cgtggtcgcg 2581 atggcaaacc cagaacggtt catggtgggg ttggacatta gcgaaaacgc tctgaagaaa 2641 gcacgcgaaa ccttttccac gatgccaaac tccagctgtt tcagcttcgt gaaggaagat 2701 gtgtttacgt ggcgtccaga gcaaccattt gacttcattt tcgattacgt cttcttctgc 2761 gccattgacc ctaaaatgcg ccctgcctgg gggaaagcga tgtatgaatt gctgaaacct 2821 gatggggagc tcatcacgct gatgtaccct attaccaacc acgagggcgg gcctccattc 2881 tcggtgtccg aatccgagta tgagaaggtg ttggtcccgc tgggtttcaa acagttgtcg 2941 ctcgaggatt actccgattt ggctgtggag cctcggaaag ggaaagagaa gctggcccgg 3001 tggaagaaga tgaacaacta aaatggtgct taaggatcca aactcgagta aggatctcca 3061 ggcatcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt 3121 tgtcggtgaa cgctctctac tagagtcaca ctggctcacc ttcgggtggg cctttctgcg 3181 aagcggcgcg ccgtcccgtc aagtcagcgt aatgctctgc cagtgttaca accaattaac 3241 caattctgat tagaaaaact catcgagcat caaatgaaac tgcaatttat tcatatcagg 3301 attatcaata ccatattttt gaaaaagccg tttctgtaat gaaggagaaa actcaccgag 3361 gcagttccat aggatggcaa gatcctggta tcggtctgcg attccgactc gtccaacatc 3421 aatacaacct attaatttcc cctcgtcaaa aataaggtta tcaagtgaga aatcaccatg 3481 agtgacgact gaatccggtg agaatggcaa aagcttatgc atttctttcc agacttgttc 3541 aacaggccag ccattacgct cgtcatcaaa atcactcgca tcaaccaaac cgttattcat 3601 tcgtgattgc gcctgagcga gacgaaatac gcgatcgctg ttaaaaggac aattacaaac 3661 aggaatcgaa tgcaaccggc gcaggaacac tgccagcgca tcaacaatat tttcacctga 3721 atcaggatat tcttctaata cctggaatgc tgttttcccg gggatcgcag tggtgagtaa 3781 ccatgcatca tcaggagtac ggataaaatg cttgatggtc ggaagaggca taaattccgt 3841 cagccagttt agtctgacca tctcatctgt aacatcattg gcaacgctac ctttgccatg 3901 tttcagaaac aactctggcg catcgggctt cccatacaat cgatagattg tcgcacctga 3961 ttgcccgaca ttatcgcgag cccatttata cccatataaa tcagcatcca tgttggaatt 4021 taatcgcggc ctcgagcaag acgtttcccg ttgaatatgg ctcataacac cccttgtatt 4081 actgtttatg taagcagaca gttttattgt tcatgatgat atatttttat cttgtgcaat 4141 gtaacatcag agattttgag acacaacgtg gctttccagg tggccggccc ttcgcaaagt 4201 cgtgaccgcc tacggcggct gcggcgccct acgggcttgc tctccgggct tcgccctgcg 4261 cggtcgctgc gctcccttgc cagcccgtgg atatgtggac gatggccgcg a //