IBMc455 1 pSB3A5*(BsaI-)-PBAD(SapI-)-B30-H6-TetR(1-70)-5aa-SYNZIP17-BCD1-SYNZIP18-5aa-TetR(71-207)-HA-Ter Creator: Trevor Y. H. Ho Principal Investigator: Baojun Wang BioSafety Level: Level 1 Backbone: pSB3A5* Origin of replication: p15A Selection Markers: Ampicillin pSB3A5*(BsaI-)-PBAD(SapI-)-B30-H6-TetR(1-70)-5aa-SYNZIP17-BCD1-SYNZIP18-5aa-TetR(71-207)-HA-Ter Expression plasmid for tagged TetR split at 70/71 with SYNZIP17 & SYNZIP18 Baojun Wang Trevor Y. H. Ho 18-NOV-2020 ds-DNA annotation7 1 SYNZIP18 #d6b295 #d6b295 SYNZIP18 range 1 2764 2889 annotation12 1 ECK120033736 #d59687 #d59687 ECK120033736 range 1 3354 3406 annotation16 1 ampR (BsaI-) #faac61 #faac61 ampR (BsaI-) range 1 3 863 annotation11 1 VF2 #75c6a9 #75c6a9 VF2 range 1 943 962 annotation0 1 araC #75c6a9 #75c6a9 araC range 1 1088 1966 annotation2 1 TetR(2-70) #b1ff67 #b1ff67 TetR(2-70) range 1 2328 2534 annotation10 1 TetR(71-207) #b1ff67 #b1ff67 TetR(71-207) range 1 2905 3315 annotation5 1 RFC10 Prefix (full) #d6b295 #d6b295 RFC10 Prefix (full) range 1 1066 1087 annotation9 1 BBa_B0030 #c7b0e3 #c7b0e3 BBa_B0030 range 1 2286 2300 annotation1 1 HA tag #d59687 #d59687 HA tag range 1 3316 3342 annotation17 1 VR #b4abac #b4abac VR range 1 3522 3541 annotation13 1 H6 #c6c9d1 #c6c9d1 H6 range 1 2310 2327 annotation15 1 RBS for CDS2 #b4abac #b4abac RBS for CDS2 range 1 2749 2757 annotation8 1 p15A origin of replication #b4abac #b4abac p15A origin of replication range 1 3988 4812 annotation4 1 SYNZIP17 #d6b295 #d6b295 SYNZIP17 range 1 2550 2675 annotation14 1 PBAD(SapI-) #faac61 #faac61 PBAD(SapI-) range 1 1994 2277 annotation18 1 RBS for CDS 1 #ffef86 #ffef86 RBS for CDS 1 range 1 2698 2705 annotation6 1 RFC10 Suffix #9eafd2 #9eafd2 RFC10 Suffix range 1 3407 3427 annotation3 1 BCD1 #008000 #008000 BCD1 range 1 2679 2763 IBMc455_seq 1 gtatgagtattcaacatttccgtgtcgcccttattcccttttttgcggcattttgccttcctgtttttgctcacccagaaacgctggtgaaagtaaaagatgctgaagatcagttgggtgcacgagtgggttatatcgaactggatctcaacagcggtaagatccttgagagttttcgccccgaagaacgttttccaatgatgagcacttttaaagttctgctatgtggcgcggtattatcccgtattgacgccgggcaagagcaactcggtcgccgcatacactattctcagaatgacttggttgagtactcaccagtcacagaaaagcatcttacggatggcatgacagtaagagaattatgcagtgctgccataaccatgagtgataacactgcggccaacttacttctgacaacgatcggaggaccgaaggagctaaccgcttttttgcacaacatgggggatcatgtaactcgccttgatcgttgggaaccggagctgaatgaagccataccaaacgacgagcgtgacaccacgatgcctgtagcaatggcaacaacgttgcgcaaactattaactggcgaactacttactctagcttcccggcaacaattaatagactggatggaggcggataaagttgcaggaccacttctgcgctcggcccttccggctggctggtttattgctgataaatctggagccggtgagcgtGGAtctcgcggtatcattgcagcactggggccagatggtaagccctcccgtatcgtagttatctacacgacggggagtcaggcaactatggatgaacgaaatagacagatcgctgagataggtgcctcactgattaagcattggtaatactagctccggcaaaaaaacgggcaaggtgtcaccaccctgccctttttctttaaaaccgaaaagattacttcgcgtttgccacctgacgtctaagaaaaggaatattcagcaatttgcccgtgccgaagaaaggcccacccgtgaaggtgagccagtgagttgattgctacgtaattagttagttagcccttagtgactcgaattcgcggccgcttctagagttatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcattttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtggtgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaaagatgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggtcgctgaaatgcggctggtgcgcttcatccgggcgaaagaaccccgtattggcaaatattgacggccagttaagccattcatgccagtaggcgcgcggacgaaagtaaacccactggtgataccattcgcgagcctccggatgacgaccgtagtgatgaatctctcctggcgggaacagcaaaatatcacccggtcggcaaacaaattctcgtccctgatttttcaccaccccctgaccgcgaatggtgagattgagaatataacctttcattcccagcggtcggtcgataaaaaaatcgagataaccgttggcctcaatcggcgttaaacccgccaccagatgggcattaaacgagtatcccggcagcaggggatcattttgcgcttcagccatacttttcatactcccgccattcagagaagaaaccaattgtccatattgcatcagacattgccgtcactgcgtcttttactggcActtctcgctaaccaaaccggtaaccccgcttattaaaagcattctgtaacaaagcgggaccaaagccatgacaaaaacgcgtaacaaaagtgtctataatcacggcagaaaagtccacattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccattactagagattaaagaggagaaatactagatgcatcatcaccatcaccactccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttaggaggtggtggatccaacgaaaaagaagaactgaaatccaaaaaagcggaactgcgcaaccgtatcgaacagctgaaacagaaacgtgaacaactgaagcagaaaatcgcgaacctgcgtaaagaaatcgaagcttacaaataaGGGCCCAAGTTCACTTAAAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAAACATCTTAATCATGCACAGGAGACTTTCTAatgagcatcgcggcgaccctggagaacgatctggcgcgtctggaaaacgaaaacgctcgtctcgaaaaagacatcgcgaacctggaacgtgacctggcgaaactggagcgtgaagaagcgtacttcggaggttcaggtggtgaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccTATCCGTATGATGTTCCGGATTATGCCtgatactagagaacgcatgagAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTtttattgcgctactagtagcggccgctgcaggagtcactaagggttagttagttagattagcagaaagtcaaaagcctccgaccggaggcttttgactaaaacttcccttggggttatcattggggctcactcaaaggcggtaatcagataaaaaaaatccttagctttcgctaaggatgatttctgctagagatggaatagactggatggaggcggataaagttgcaggaccacttctgcgctcggcccttccggctggctggtttattgctgataaatctggagccggtgagcgtggatctcgcggtatcattgcagcactggggccagatggtaagccctcccgtatcgtagttatctacacgacggggagtcaggcaactatggatgaacgaaatagacagatcgctgagataggtgcctcactgattaagcattggtaactgtcagaccaagtttactcatatatactttagattgatttaaaacttcatttttaatttaaaaggatctaggtgaagatcctttttgataatctcatgaccaaaatcccttaacgtgagttttcgttccactgagcgtcagaccccttaataagatgatcttcttgagatcgttttggtctgcgcgtaatctcttgctctgaaaacgaaaaaaccgccttgcagggcggtttttcgaaggttctctgagctaccaactctttgaaccgaggtaactggcttggaggagcgcagtcaccaaaacttgtcctttcagtttagccttaaccggcgcatgacttcaagactaactcctctaaatcaattaccagtggctgctgccagtggtgcttttgcatgtctttccgggttggactcaagacgatagttaccggataaggcgcagcggtcggactgaacggggggttcgtgcatacagtccagcttggagcgaactgcctacccggaactgagtgtcaggcgtggaatgagacaaacgcggccataacagcggaatgacaccggtaaaccgaaaggcaggaacaggagagcgcacgagggagccgccaggggaaacgcctggtatctttatagtcctgtcgggtttcgccaccactgatttgagcgtcagatttcgtgatgcttgtcaggggggcggagcctatggaaaaacggctttgccgcggccctctcacttccctgttaagtatcttcctggcatcttccaggaaatctccgccccgttcgtaagccatttccgctcgccgcagtcgaacgaccgagcgtagcgagtcagtgagcgaggaagcggaatatatcctgtatcacatattctgctgacgcaccggtgcagccttttttctcctgccacatgaagcacttcactgacaccctcatcagtgccaacatagtaagccagtatacactccgctagcgctgaggtctgcctcgtgaagaaggtgttgctgactcataccaggcctgaatcgccccatcatccagccagaaagtgagggagccacggttgatgagagctttgttgtaggtggaccagttggtgattttgaacttttgctttgccacggaacggtctgcgttgtcgggaagatgcgtgatctgatccttcaactcagcaaaagttcgatttattcaacaaagccacgttgtgtctcaaaatctctgatgttacattgcacaagataaaaatatatcatcatgaacaataaaactgtctgcttacataaacagtaatacaaggggtgtttactagag