LOCUS IBMc452 4925 bp ds-DNA circular 18-NOV-2020 DEFINITION pSB3T5(BsaI-)-PPhlF-RiboJ-BCD1-SYNZIP18-5aa-mCherry*(192-236)-H6-Te r ACCESSION IBMc452 VERSION IBMc452.1 FEATURES Location/Qualifiers terminator 1516..1576 /ApEinfo_fwdcolor="#84b0dc" /ApEinfo_revcolor="#84b0dc" /label="L3S2P21" CDS 2831..2956 /ApEinfo_fwdcolor="#d6b295" /ApEinfo_revcolor="#d6b295" /label="SYNZIP18" misc_feature 1494..1515 /ApEinfo_fwdcolor="#d6b295" /ApEinfo_revcolor="#d6b295" /label="RFC10 Prefix (full)" RBS 2816..2824 /ApEinfo_fwdcolor="#b4abac" /ApEinfo_revcolor="#b4abac" /label="RBS for CDS2" primer_bind 1371..1390 /ApEinfo_fwdcolor="#75c6a9" /ApEinfo_revcolor="#75c6a9" /label="VF2" RBS 2765..2772 /ApEinfo_fwdcolor="#ffef86" /ApEinfo_revcolor="#ffef86" /label="RBS for CDS 1" CDS 98..1291 /ApEinfo_fwdcolor="#9eafd2" /ApEinfo_revcolor="#9eafd2" /label="tetR" misc_feature 2668..2742 /ApEinfo_fwdcolor="#b1ff67" /ApEinfo_revcolor="#b1ff67" /label="RiboJ" /featureType="insulator" promoter 9..97 /ApEinfo_fwdcolor="#f58a5e" /ApEinfo_revcolor="#f58a5e" /label="tetR promoter" promoter 2602..2667 /ApEinfo_fwdcolor="#f58a5e" /ApEinfo_revcolor="#f58a5e" /label="PPhlF" primer_bind complement(3304..3323) /ApEinfo_fwdcolor="#b4abac" /ApEinfo_revcolor="#b4abac" /label="VR" misc_feature 2746..2830 /ApEinfo_fwdcolor="#008000" /ApEinfo_revcolor="#008000" /label="BCD1" terminator 2530..2582 /ApEinfo_fwdcolor="#84b0dc" /ApEinfo_revcolor="#84b0dc" /label="L3S3P21" rep_origin 3770..4594 /ApEinfo_fwdcolor="#b4abac" /ApEinfo_revcolor="#b4abac" /label="p15A origin of replication" terminator 2275..2529 /ApEinfo_fwdcolor="#84b0dc" /ApEinfo_revcolor="#84b0dc" /label="IOT" misc_feature 3189..3209 /ApEinfo_fwdcolor="#9eafd2" /ApEinfo_revcolor="#9eafd2" /label="RFC10 Suffix" CDS 1663..2265 /ApEinfo_fwdcolor="#b7e6d7" /ApEinfo_revcolor="#b7e6d7" /label="PhlF" terminator 3136..3188 /ApEinfo_fwdcolor="#d59687" /ApEinfo_revcolor="#d59687" /label="ECK120033736" RBS 1635..1662 /ApEinfo_fwdcolor="#ff9ccd" /ApEinfo_revcolor="#ff9ccd" /label="phl2" CDS 3107..3124 /ApEinfo_fwdcolor="#c6c9d1" /ApEinfo_revcolor="#c6c9d1" /label="H6" promoter 1581..1634 /ApEinfo_fwdcolor="#d59687" /ApEinfo_revcolor="#d59687" /label="PlacIQ" CDS 2972..3106 /ApEinfo_fwdcolor="#faac61" /ApEinfo_revcolor="#faac61" /label="mCherry*(192-236)" ORIGIN 1 tactagagga gattctcatg tttgacagct tatcatcgat aagctttaat gcggtagttt 61 atcacagtta aattgctaac gcagtcaggc accgtgtatg aaatctaaca atgcgctcat 121 cgtcattctc ggcaccgtca ccctggacgc tgtaggcata ggcttggtta tgccggtact 181 gccgggcctc ttgcgggata tcgtccattc cgacagtatt gccagtcact atggcgtgct 241 gcttgcgctc tatgcgttga tgcaatttct ttgcgcaccc gttctcggag ccctgtccga 301 ccgctttggc cgccgtccag tcctgctcgc ttcgctcctt ggagccacta tcgactacgc 361 gatcatggcg accacacccg tcctgtggat tctctacgcc ggacgcatcg tggcgggcat 421 cacgggtgcc acaggtgcgg ttgctggtgc ctatatcgcc gacatcaccg atggggaaga 481 tcgggctcgc cacttcgggc tcatgagcgc ttgtttcggc gtgggtatgg tggcaggccc 541 cgtggccggg ggactgttgg gtgccatctc cttgcatgca ccattccttg cggcggcggt 601 gctcaacggc ctcaacctcc tcctgggctg cttccttatg caggaatcgc ataagggaga 661 gcgccgtccg atgcccttgc gtgccttcaa tccagtcagc tccttccggt gggcgcgggg 721 catgactatc gtcgccgcac ttatgactgt tttctttatc atgcaactcg taggacaggt 781 tccggcagcg ctctgggtca ttttcggcga ggaccgcttt cgctggagcg cgacgatgat 841 cggcctgtcg cttgcggtat tcggaatctt gcacgccctc gctcaagcct tcgtcacggg 901 ccccgccacc aaacgtttcg gcgagaagca ggccattatc gcgggcatgg cggccgacgc 961 gctgggctac gtcttgctgg cgttcgcgac gcgcggctgg atggccttcc ccattatgat 1021 tcttctcgct tccggcggca tcggtatgcc cgcgttgcag gccatgctgt cccgccaagt 1081 agatgacgac catcagggac agcttcaagg gtcgctcgcg gctcttacca gcctcacttc 1141 gatcattgga ccgctgatcg tcacggcgat ttatgccgcc tcggcgagca catggaacgg 1201 gttggcatgg attgtaggtg ccgcccttta ccttgtctgc ctccccgcgt tgcgtcgcgg 1261 tgcatggagc cgggccacct cgacctaata atactagctc cggcaaaaaa acgggcaagg 1321 tgtcaccacc ctgccctttt tctttaaaac cgaaaagatt acttcgcgtt tgccacctga 1381 cgtctaagaa aaggaatatt cagcaatttg cccgtgccga agaaaggccc acccgtgaag 1441 gtgagccagt gagttgattg ctacgtaatt agttagttag cccttagtga ctcgaattcg 1501 cggccgcttc tagagctcgg taccaaattc cagaaaagag gcctcccgaa aggggggcct 1561 tttttcgttt tggtccaatg gcggcgcgcc atcgaatggt gcaaaacctt tcgcggtatg 1621 gcatgatagc gcccggaaga gagtcaattc atgggggtga atatggcacg taccccgagc 1681 cgtagcagca ttggtagcct gcgtagtccg catacccata aagcaattct gaccagcacc 1741 attgaaatcc tgaaagaatg tggttatagc ggtctgagca ttgaaagcgt ggcacgtcgc 1801 gccggtgcag gcaaaccgac catttatcgt tggtggacca acaaagcagc actgattgcc 1861 gaagtgtatg aaaatgaaat cgaacaggta cgtaaatttc cggatttggg tagctttaaa 1921 gccgatctgg attttctgct gcataatctg tggaaagttt ggcgtgaaac catttgtggt 1981 gaagcatttc gttgtgttat tgcagaagca cagttggacc ctgtaaccct gacccaactg 2041 aaagatcagt ttatggaacg tcgtcgtgag ataccgaaaa aactggttga agatgccatt 2101 agcaatggtg aactgccgaa agatatcaat cgtgaactgc tgctggatat gatttttggt 2161 ttttgttggt atcgcctgct gaccgaacag ttgaccgttg aacaggatat tgaagaattt 2221 accttcctgc tgattaatgg tgtttgtccg ggtacacagt gttgataagg atcctaattg 2281 gtaacgaatc agacaattga cggctcgagg gagtagcata gggtttgcag aatccctgct 2341 tcgtccattt gacaggcaca ttatgcatcg atgataagct gtcaaacatg agcagatcct 2401 ctacgccgga cgcatcgtgg ccggcatcac cggcgccaca ggtgcggttg ctggcgccta 2461 tatcgccgac atcaccgatg gggaagatcg ggctcgccac ttcgggctca tgagcaaata 2521 ttttatctgc caattattga aggcctccct aacggggggc ctttttttgt ttctggtctc 2581 ccgcttaacg atcgttggct gcgacgtacg gtggaatctg attcgttacc aattgacatg 2641 atacgaaacg taccgtatcg ttaaggtagc tgtcaccgga tgtgctttcc ggtctgatga 2701 gtccgtgagg acgaaacagc ctctacaaat aattttgttt aataaGGGCC CAAGTTCACT 2761 TAAAAAGGAG ATCAACAATG AAAGCAATTT TCGTACTGAA ACATCTTAAT CATGCACAGG 2821 AGACTTTCTA atgagcatcg cggcgaccct ggagaacgat ctggcgcgtc tggaaaacga 2881 aaacgctcgt ctcgaaaaag acatcgcgaa cctggaacgt gacctggcga aactggagcg 2941 tgaagaagcg tacttcggag gttcaggtgg tgtgcagctg cccggcgcct acaacgtcaa 3001 catcaagttg gacatcacct cccacaacga ggactacacc atcgtggaac agtacgaacg 3061 cgccgagggc cgccactcca ccggcggcat ggacgagctg tacaagcatc atcaccatca 3121 ccactaatac tagagaacgc atgagAAAGC CCCCGGAAGA TCACCTTCCG GGGGCTTTtt 3181 tattgcgcta ctagtagcgg ccgctgcagg agtcactaag ggttagttag ttagattagc 3241 agaaagtcaa aagcctccga ccggaggctt ttgactaaaa cttcccttgg ggttatcatt 3301 ggggctcact caaaggcggt aatcagataa aaaaaatcct tagctttcgc taaggatgat 3361 ttctgctaga gatggaatag actggatgga ggcggataaa gttgcaggac cacttctgcg 3421 ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtggatc 3481 tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta 3541 cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc 3601 ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga 3661 tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat 3721 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagacccct taataagatg 3781 atcttcttga gatcgttttg gtctgcgcgt aatctcttgc tctgaaaacg aaaaaaccgc 3841 cttgcagggc ggtttttcga aggttctctg agctaccaac tctttgaacc gaggtaactg 3901 gcttggagga gcgcagtcac caaaacttgt cctttcagtt tagccttaac cggcgcatga 3961 cttcaagact aactcctcta aatcaattac cagtggctgc tgccagtggt gcttttgcat 4021 gtctttccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcggactgaa 4081 cggggggttc gtgcatacag tccagcttgg agcgaactgc ctacccggaa ctgagtgtca 4141 ggcgtggaat gagacaaacg cggccataac agcggaatga caccggtaaa ccgaaaggca 4201 ggaacaggag agcgcacgag ggagccgcca ggggaaacgc ctggtatctt tatagtcctg 4261 tcgggtttcg ccaccactga tttgagcgtc agatttcgtg atgcttgtca ggggggcgga 4321 gcctatggaa aaacggcttt gccgcggccc tctcacttcc ctgttaagta tcttcctggc 4381 atcttccagg aaatctccgc cccgttcgta agccatttcc gctcgccgca gtcgaacgac 4441 cgagcgtagc gagtcagtga gcgaggaagc ggaatatatc ctgtatcaca tattctgctg 4501 acgcaccggt gcagcctttt ttctcctgcc acatgaagca cttcactgac accctcatca 4561 gtgccaacat agtaagccag tatacactcc gctagcgctg aggtctgcct cgtgaagaag 4621 gtgttgctga ctcataccag gcctgaatcg ccccatcatc cagccagaaa gtgagggagc 4681 cacggttgat gagagctttg ttgtaggtgg accagttggt gattttgaac ttttgctttg 4741 ccacggaacg gtctgcgttg tcgggaagat gcgtgatctg atccttcaac tcagcaaaag 4801 ttcgatttat tcaacaaagc cacgttgtgt ctcaaaatct ctgatgttac attgcacaag 4861 ataaaaatat atcatcatga acaataaaac tgtctgctta cataaacagt aatacaaggg 4921 gtgtt //