LOCUS IBMc333 4700 bp ds-DNA circular 18-NOV-2020 DEFINITION pSB3T5(BsaI-)-PBAD(SapI-)-B30-SrpR(1-22)-BsaI-BsaI-SrpR(192-213)-Te r ACCESSION IBMc333 VERSION IBMc333.1 FEATURES Location/Qualifiers RBS 2714..2728 /ApEinfo_fwdcolor="#c7b0e3" /ApEinfo_revcolor="#c7b0e3" /label="BBa_B0030" primer_bind complement(3079..3098) /ApEinfo_fwdcolor="#b4abac" /ApEinfo_revcolor="#b4abac" /label="VR" terminator 2911..2963 /ApEinfo_fwdcolor="#d59687" /ApEinfo_revcolor="#d59687" /label="ECK120033736" misc_feature 2810..2825 /ApEinfo_fwdcolor="#d6b295" /ApEinfo_revcolor="#d6b295" /label="spacing sequence" promoter 2422..2705 /ApEinfo_fwdcolor="#faac61" /ApEinfo_revcolor="#faac61" /label="PBAD(SapI-)" CDS 98..1291 /ApEinfo_fwdcolor="#9eafd2" /ApEinfo_revcolor="#9eafd2" /label="tetR" misc_feature 2964..2984 /ApEinfo_fwdcolor="#9eafd2" /ApEinfo_revcolor="#9eafd2" /label="RFC10 Suffix" misc_feature 1494..1515 /ApEinfo_fwdcolor="#d6b295" /ApEinfo_revcolor="#d6b295" /label="RFC10 Prefix (full)" rep_origin 3545..4369 /ApEinfo_fwdcolor="#b4abac" /ApEinfo_revcolor="#b4abac" /label="p15A origin of replication" promoter 9..97 /ApEinfo_fwdcolor="#f58a5e" /ApEinfo_revcolor="#f58a5e" /label="tetR promoter" CDS complement(1516..2394) /ApEinfo_fwdcolor="#75c6a9" /ApEinfo_revcolor="#75c6a9" /label="araC" primer_bind 1371..1390 /ApEinfo_fwdcolor="#75c6a9" /ApEinfo_revcolor="#75c6a9" /label="VF2" CDS 2833..2899 /ApEinfo_fwdcolor="#75c6a9" /ApEinfo_revcolor="#75c6a9" /label="SrpR(192-213)" CDS 2735..2802 /ApEinfo_fwdcolor="#75c6a9" /ApEinfo_revcolor="#75c6a9" /label="SrpR(1-22)" ORIGIN 1 tactagagga gattctcatg tttgacagct tatcatcgat aagctttaat gcggtagttt 61 atcacagtta aattgctaac gcagtcaggc accgtgtatg aaatctaaca atgcgctcat 121 cgtcattctc ggcaccgtca ccctggacgc tgtaggcata ggcttggtta tgccggtact 181 gccgggcctc ttgcgggata tcgtccattc cgacagtatt gccagtcact atggcgtgct 241 gcttgcgctc tatgcgttga tgcaatttct ttgcgcaccc gttctcggag ccctgtccga 301 ccgctttggc cgccgtccag tcctgctcgc ttcgctcctt ggagccacta tcgactacgc 361 gatcatggcg accacacccg tcctgtggat tctctacgcc ggacgcatcg tggcgggcat 421 cacgggtgcc acaggtgcgg ttgctggtgc ctatatcgcc gacatcaccg atggggaaga 481 tcgggctcgc cacttcgggc tcatgagcgc ttgtttcggc gtgggtatgg tggcaggccc 541 cgtggccggg ggactgttgg gtgccatctc cttgcatgca ccattccttg cggcggcggt 601 gctcaacggc ctcaacctcc tcctgggctg cttccttatg caggaatcgc ataagggaga 661 gcgccgtccg atgcccttgc gtgccttcaa tccagtcagc tccttccggt gggcgcgggg 721 catgactatc gtcgccgcac ttatgactgt tttctttatc atgcaactcg taggacaggt 781 tccggcagcg ctctgggtca ttttcggcga ggaccgcttt cgctggagcg cgacgatgat 841 cggcctgtcg cttgcggtat tcggaatctt gcacgccctc gctcaagcct tcgtcacggg 901 ccccgccacc aaacgtttcg gcgagaagca ggccattatc gcgggcatgg cggccgacgc 961 gctgggctac gtcttgctgg cgttcgcgac gcgcggctgg atggccttcc ccattatgat 1021 tcttctcgct tccggcggca tcggtatgcc cgcgttgcag gccatgctgt cccgccaagt 1081 agatgacgac catcagggac agcttcaagg gtcgctcgcg gctcttacca gcctcacttc 1141 gatcattgga ccgctgatcg tcacggcgat ttatgccgcc tcggcgagca catggaacgg 1201 gttggcatgg attgtaggtg ccgcccttta ccttgtctgc ctccccgcgt tgcgtcgcgg 1261 tgcatggagc cgggccacct cgacctaata atactagctc cggcaaaaaa acgggcaagg 1321 tgtcaccacc ctgccctttt tctttaaaac cgaaaagatt acttcgcgtt tgccacctga 1381 cgtctaagaa aaggaatatt cagcaatttg cccgtgccga agaaaggccc acccgtgaag 1441 gtgagccagt gagttgattg ctacgtaatt agttagttag cccttagtga ctcgaattcg 1501 cggccgcttc tagagttatg acaacttgac ggctacatca ttcacttttt cttcacaacc 1561 ggcacggaac tcgctcgggc tggccccggt gcatttttta aatacccgcg agaaatagag 1621 ttgatcgtca aaaccaacat tgcgaccgac ggtggcgata ggcatccggg tggtgctcaa 1681 aagcagcttc gcctggctga tacgttggtc ctcgcgccag cttaagacgc taatccctaa 1741 ctgctggcgg aaaagatgtg acagacgcga cggcgacaag caaacatgct gtgcgacgct 1801 ggcgatatca aaattgctgt ctgccaggtg atcgctgatg tactgacaag cctcgcgtac 1861 ccgattatcc atcggtggat ggagcgactc gttaatcgct tccatgcgcc gcagtaacaa 1921 ttgctcaagc agatttatcg ccagcagctc cgaatagcgc ccttcccctt gcccggcgtt 1981 aatgatttgc ccaaacaggt cgctgaaatg cggctggtgc gcttcatccg ggcgaaagaa 2041 ccccgtattg gcaaatattg acggccagtt aagccattca tgccagtagg cgcgcggacg 2101 aaagtaaacc cactggtgat accattcgcg agcctccgga tgacgaccgt agtgatgaat 2161 ctctcctggc gggaacagca aaatatcacc cggtcggcaa acaaattctc gtccctgatt 2221 tttcaccacc ccctgaccgc gaatggtgag attgagaata taacctttca ttcccagcgg 2281 tcggtcgata aaaaaatcga gataaccgtt ggcctcaatc ggcgttaaac ccgccaccag 2341 atgggcatta aacgagtatc ccggcagcag gggatcattt tgcgcttcag ccatactttt 2401 catactcccg ccattcagag aagaaaccaa ttgtccatat tgcatcagac attgccgtca 2461 ctgcgtcttt tactggcAct tctcgctaac caaaccggta accccgctta ttaaaagcat 2521 tctgtaacaa agcgggacca aagccatgac aaaaacgcgt aacaaaagtg tctataatca 2581 cggcagaaaa gtccacattg attatttgca cggcgtcaca ctttgctatg ccatagcatt 2641 tttatccata agattagcgg atcctacctg acgcttttta tcgcaactct ctactgtttc 2701 tccattacta gagattaaag aggagaaata ctagATGGCA CGTAAAACCG CAGCAGAAGC 2761 AGAAGAAACC CGTCAGCGTA TTATTGATGC AGCACTGGAA GTTGAGACCa cggccacatc 2821 gtggaGGTCT CAGCTGCGTG AACCGCCTCG TTTTCTGCTG TGTGAAGAAG CACAGATTAA 2881 ACAGGTGAAA TCCTTCGAAT AAtactagag aacgcatgag AAAGCCCCCG GAAGATCACC 2941 TTCCGGGGGC TTTtttattg cgctactagt agcggccgct gcaggagtca ctaagggtta 3001 gttagttaga ttagcagaaa gtcaaaagcc tccgaccgga ggcttttgac taaaacttcc 3061 cttggggtta tcattggggc tcactcaaag gcggtaatca gataaaaaaa atccttagct 3121 ttcgctaagg atgatttctg ctagagatgg aatagactgg atggaggcgg ataaagttgc 3181 aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc 3241 cggtgagcgt ggatctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg 3301 tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat 3361 cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata 3421 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct 3481 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga 3541 ccccttaata agatgatctt cttgagatcg ttttggtctg cgcgtaatct cttgctctga 3601 aaacgaaaaa accgccttgc agggcggttt ttcgaaggtt ctctgagcta ccaactcttt 3661 gaaccgaggt aactggcttg gaggagcgca gtcaccaaaa cttgtccttt cagtttagcc 3721 ttaaccggcg catgacttca agactaactc ctctaaatca attaccagtg gctgctgcca 3781 gtggtgcttt tgcatgtctt tccgggttgg actcaagacg atagttaccg gataaggcgc 3841 agcggtcgga ctgaacgggg ggttcgtgca tacagtccag cttggagcga actgcctacc 3901 cggaactgag tgtcaggcgt ggaatgagac aaacgcggcc ataacagcgg aatgacaccg 3961 gtaaaccgaa aggcaggaac aggagagcgc acgagggagc cgccagggga aacgcctggt 4021 atctttatag tcctgtcggg tttcgccacc actgatttga gcgtcagatt tcgtgatgct 4081 tgtcaggggg gcggagccta tggaaaaacg gctttgccgc ggccctctca cttccctgtt 4141 aagtatcttc ctggcatctt ccaggaaatc tccgccccgt tcgtaagcca tttccgctcg 4201 ccgcagtcga acgaccgagc gtagcgagtc agtgagcgag gaagcggaat atatcctgta 4261 tcacatattc tgctgacgca ccggtgcagc cttttttctc ctgccacatg aagcacttca 4321 ctgacaccct catcagtgcc aacatagtaa gccagtatac actccgctag cgctgaggtc 4381 tgcctcgtga agaaggtgtt gctgactcat accaggcctg aatcgcccca tcatccagcc 4441 agaaagtgag ggagccacgg ttgatgagag ctttgttgta ggtggaccag ttggtgattt 4501 tgaacttttg ctttgccacg gaacggtctg cgttgtcggg aagatgcgtg atctgatcct 4561 tcaactcagc aaaagttcga tttattcaac aaagccacgt tgtgtctcaa aatctctgat 4621 gttacattgc acaagataaa aatatatcat catgaacaat aaaactgtct gcttacataa 4681 acagtaatac aaggggtgtt //