LOCUS IBMc187 6598 bp ds-DNA circular 18-NOV-2020 DEFINITION pSB3T5(BsaI-)-PBAD(SapI-)-B33-ECF20(1-101)-T-cat-PBAD(SapI-)-RiboJ- BCD22-ECF20(102-193)-T ACCESSION IBMc187 VERSION IBMc187.1 FEATURES Location/Qualifiers terminator 4809..4861 /ApEinfo_fwdcolor="#d59687" /ApEinfo_revcolor="#d59687" /label="ECK120033736" RBS 2714..2724 /ApEinfo_fwdcolor="#ff9ccd" /ApEinfo_revcolor="#ff9ccd" /label="BBa_B0033" CDS 4522..4797 /ApEinfo_fwdcolor="#ffef86" /ApEinfo_revcolor="#ffef86" /label="ECF20 102-193" promoter 2422..2705 /ApEinfo_fwdcolor="#faac61" /ApEinfo_revcolor="#faac61" /label="PBAD(SapI-)" RBS 4504..4512 /ApEinfo_fwdcolor="#85dae9" /ApEinfo_revcolor="#85dae9" /label="RBS for 2nd CDS" CDS complement(1516..2394) /ApEinfo_fwdcolor="#75c6a9" /ApEinfo_revcolor="#75c6a9" /label="araC" RBS 4453..4460 /ApEinfo_fwdcolor="#ffef86" /ApEinfo_revcolor="#ffef86" /label="RBS for CDS 1" misc_feature 1494..1515 /ApEinfo_fwdcolor="#d6b295" /ApEinfo_revcolor="#d6b295" /label="RFC10 Prefix (full)" misc_feature 4434..4521 /ApEinfo_fwdcolor="#faac61" /ApEinfo_revcolor="#faac61" /label="BCD22" primer_bind 1371..1390 /ApEinfo_fwdcolor="#75c6a9" /ApEinfo_revcolor="#75c6a9" /label="VF2" promoter 4070..4353 /ApEinfo_fwdcolor="#faac61" /ApEinfo_revcolor="#faac61" /label="PBAD(SapI-)" CDS 98..1291 /ApEinfo_fwdcolor="#9eafd2" /ApEinfo_revcolor="#9eafd2" /label="tetR" terminator 3994..4050 /ApEinfo_fwdcolor="#ff0000" /ApEinfo_revcolor="#ff0000" /label="L3S2P55" promoter 9..97 /ApEinfo_fwdcolor="#f58a5e" /ApEinfo_revcolor="#f58a5e" /label="tetR promoter" CDS complement(3228..3887) /ApEinfo_fwdcolor="#ff9ccd" /ApEinfo_revcolor="#ff9ccd" /label="cat (BsmBI-)" primer_bind complement(4977..4996) /ApEinfo_fwdcolor="#b4abac" /ApEinfo_revcolor="#b4abac" /label="VR" promoter complement(3888..3985) /ApEinfo_fwdcolor="#c6c9d1" /ApEinfo_revcolor="#c6c9d1" /label="cat promoter" terminator complement(3143..3199) /ApEinfo_fwdcolor="#d59687" /ApEinfo_revcolor="#d59687" /label="ECK120033737" rep_origin 5443..6267 /ApEinfo_fwdcolor="#b4abac" /ApEinfo_revcolor="#b4abac" /label="p15A origin of replication" terminator 3045..3134 /ApEinfo_fwdcolor="#f58a5e" /ApEinfo_revcolor="#f58a5e" /label="ECK120029600" misc_feature 4862..4882 /ApEinfo_fwdcolor="#9eafd2" /ApEinfo_revcolor="#9eafd2" /label="RFC10 Suffix" CDS 2731..3033 /ApEinfo_fwdcolor="#ffef86" /ApEinfo_revcolor="#ffef86" /label="ECF20 1-101" ORIGIN 1 tactagagga gattctcatg tttgacagct tatcatcgat aagctttaat gcggtagttt 61 atcacagtta aattgctaac gcagtcaggc accgtgtatg aaatctaaca atgcgctcat 121 cgtcattctc ggcaccgtca ccctggacgc tgtaggcata ggcttggtta tgccggtact 181 gccgggcctc ttgcgggata tcgtccattc cgacagtatt gccagtcact atggcgtgct 241 gcttgcgctc tatgcgttga tgcaatttct ttgcgcaccc gttctcggag ccctgtccga 301 ccgctttggc cgccgtccag tcctgctcgc ttcgctcctt ggagccacta tcgactacgc 361 gatcatggcg accacacccg tcctgtggat tctctacgcc ggacgcatcg tggcgggcat 421 cacgggtgcc acaggtgcgg ttgctggtgc ctatatcgcc gacatcaccg atggggaaga 481 tcgggctcgc cacttcgggc tcatgagcgc ttgtttcggc gtgggtatgg tggcaggccc 541 cgtggccggg ggactgttgg gtgccatctc cttgcatgca ccattccttg cggcggcggt 601 gctcaacggc ctcaacctcc tcctgggctg cttccttatg caggaatcgc ataagggaga 661 gcgccgtccg atgcccttgc gtgccttcaa tccagtcagc tccttccggt gggcgcgggg 721 catgactatc gtcgccgcac ttatgactgt tttctttatc atgcaactcg taggacaggt 781 tccggcagcg ctctgggtca ttttcggcga ggaccgcttt cgctggagcg cgacgatgat 841 cggcctgtcg cttgcggtat tcggaatctt gcacgccctc gctcaagcct tcgtcacggg 901 ccccgccacc aaacgtttcg gcgagaagca ggccattatc gcgggcatgg cggccgacgc 961 gctgggctac gtcttgctgg cgttcgcgac gcgcggctgg atggccttcc ccattatgat 1021 tcttctcgct tccggcggca tcggtatgcc cgcgttgcag gccatgctgt cccgccaagt 1081 agatgacgac catcagggac agcttcaagg gtcgctcgcg gctcttacca gcctcacttc 1141 gatcattgga ccgctgatcg tcacggcgat ttatgccgcc tcggcgagca catggaacgg 1201 gttggcatgg attgtaggtg ccgcccttta ccttgtctgc ctccccgcgt tgcgtcgcgg 1261 tgcatggagc cgggccacct cgacctaata atactagctc cggcaaaaaa acgggcaagg 1321 tgtcaccacc ctgccctttt tctttaaaac cgaaaagatt acttcgcgtt tgccacctga 1381 cgtctaagaa aaggaatatt cagcaatttg cccgtgccga agaaaggccc acccgtgaag 1441 gtgagccagt gagttgattg ctacgtaatt agttagttag cccttagtga ctcgaattcg 1501 cggccgcttc tagagttatg acaacttgac ggctacatca ttcacttttt cttcacaacc 1561 ggcacggaac tcgctcgggc tggccccggt gcatttttta aatacccgcg agaaatagag 1621 ttgatcgtca aaaccaacat tgcgaccgac ggtggcgata ggcatccggg tggtgctcaa 1681 aagcagcttc gcctggctga tacgttggtc ctcgcgccag cttaagacgc taatccctaa 1741 ctgctggcgg aaaagatgtg acagacgcga cggcgacaag caaacatgct gtgcgacgct 1801 ggcgatatca aaattgctgt ctgccaggtg atcgctgatg tactgacaag cctcgcgtac 1861 ccgattatcc atcggtggat ggagcgactc gttaatcgct tccatgcgcc gcagtaacaa 1921 ttgctcaagc agatttatcg ccagcagctc cgaatagcgc ccttcccctt gcccggcgtt 1981 aatgatttgc ccaaacaggt cgctgaaatg cggctggtgc gcttcatccg ggcgaaagaa 2041 ccccgtattg gcaaatattg acggccagtt aagccattca tgccagtagg cgcgcggacg 2101 aaagtaaacc cactggtgat accattcgcg agcctccgga tgacgaccgt agtgatgaat 2161 ctctcctggc gggaacagca aaatatcacc cggtcggcaa acaaattctc gtccctgatt 2221 tttcaccacc ccctgaccgc gaatggtgag attgagaata taacctttca ttcccagcgg 2281 tcggtcgata aaaaaatcga gataaccgtt ggcctcaatc ggcgttaaac ccgccaccag 2341 atgggcatta aacgagtatc ccggcagcag gggatcattt tgcgcttcag ccatactttt 2401 catactcccg ccattcagag aagaaaccaa ttgtccatat tgcatcagac attgccgtca 2461 ctgcgtcttt tactggcAct tctcgctaac caaaccggta accccgctta ttaaaagcat 2521 tctgtaacaa agcgggacca aagccatgac aaaaacgcgt aacaaaagtg tctataatca 2581 cggcagaaaa gtccacattg attatttgca cggcgtcaca ctttgctatg ccatagcatt 2641 tttatccata agattagcgg atcctacctg acgcttttta tcgcaactct ctactgtttc 2701 tccattacta gagtcacaca ggactactag atgaatgaaa ccgatcctga tctggaactg 2761 ctgaaacgta ttggtaataa tgatgcacag gccgttaaag aaatggttac ccgtaaactg 2821 cctcgtctgc tggcactggc aagtcgcctg ctgggtgatg cagatgaagc acgtgatatt 2881 gcacaagaaa gttttctgcg catttggaaa caggcagcaa gctggcgtag cgaacaggca 2941 cgttttgata cctggctgca tcgtgttgca ctgaatctgt gttatgatcg tctgcgtcgt 3001 cgtaaagaac atgtgccggt tgatagcgaa cattaaTACT AGAGttgaga agagaaaaga 3061 aaaccgccga tcctgtccac cgcattactg caaggtagtg gacaagaccg gcggtcttaa 3121 gttttttggc tgaaTACTAG AGcctttggt cgAAAAAAAA AGCCCGCACT GTCAGGTGCG 3181 GGCTTTTTTc tgtgtttccT ACTAGAGCAA GCGAGCTCGA TATCAAATTA CGCCCCGCCC 3241 TGCCACTCAT CGCAGTACTG TTGTAATTCA TTAAGCATTC TGCCGACATG GAAGCCATCA 3301 CAAACGGCAT GATGAACCTG AATCGCCAGC GGCATCAGCA CCTTGTCGCC TTGCGTATAA 3361 TATTTGCCCA TGGTGAAAAC GGGGGCGAAG AAGTTGTCCA TATTGGCCAC GTTTAAATCA 3421 AAACTGGTGA AACTCACCCA GGGATTGGCT GAAACGAAAA ACATATTCTC AATAAACCCT 3481 TTAGGGAAAT AGGCCAGGTT TTCACCGTAA CACGCCACAT CTTGCGAATA TATGTGTAGA 3541 AACTGCCGGA AATCGTCGTG GTATTCACTC CAGAGCGATG AAAACGTTTC AGTTTGCTCA 3601 TGGAAAACGG TGTAACAAGG GTGAACACTA TCCCATATCA CCAGCTCACC GTCTTTCATT 3661 GCCATACGAA ATTCCGGATG AGCATTCATC AGGCGGGCAA GAATGTGAAT AAAGGCCGGA 3721 TAAAACTTGT GCTTATTTTT CTTTACGGTC TTTAAAAAGG CCGTAATATC CAGCTGAACG 3781 GTCTGGTTAT AGGTACATTG AGCAACTGAC TGAAATGCCT CAAAATGTTC TTTACGATGC 3841 CATTGGGATA TATCAACGGT GGTATATCCA GTGATTTTTT TCTCCATTTT AGCTTCCTTA 3901 GCTCCTGAAA ATCTCGATAA CTCAAAAAAT ACGCCCGGTA GTGATCTTAT TTCATTATGG 3961 TGAAAGTTGG AACCTCTTAC GTGCCTACTA GAGCTCGGTA CCAAAGACGA ACAATAAGAC 4021 GCTGAAAAGC GTCTTTTTTC GTTTTGGTCC gcttaacgat cgttggctga gaaaccaatt 4081 gtccatattg catcagacat tgccgtcact gcgtctttta ctggcActtc tcgctaacca 4141 aaccggtaac cccgcttatt aaaagcattc tgtaacaaag cgggaccaaa gccatgacaa 4201 aaacgcgtaa caaaagtgtc tataatcacg gcagaaaagt ccacattgat tatttgcacg 4261 gcgtcacact ttgctatgcc atagcatttt tatccataag attagcggat cctacctgac 4321 gctttttatc gcaactctct actgtttctc catacccgag ctgtcaccgg atgtgctttc 4381 cggtctgatg agtccgtgag gacgaaacag cctctacaaa taattttgtt taaGGGCCCA 4441 AGTTCACTTA AAAAGGAGAT CAACAATGAA AGCAATTTTC GTACTGAAAC ATCTTAATCA 4501 TGCCTAGGAA GTTTTCTAAT Ggcctgtgaa gcactggata cccgtccggc accggatgaa 4561 cagctggaag caagcgcaca gagccgtcgt atggcacagg cactggatca gctgccggat 4621 cgtcagcgtg aagcaattgt tctgcagtat tatcaagaac tgagcaatac cgaagcagca 4681 gcactgatgc aaattagcgt tgaagccctg gaaagcctgc tgagccgtgc acgtcgtaat 4741 ctgcgtagcc atctggccga agcaccgggt gcagatctga gcggtcgtcg caaaccgtaa 4801 tactagagaa cgcatgagAA AGCCCCCGGA AGATCACCTT CCGGGGGCTT Ttttattgcg 4861 ctactagtag cggccgctgc aggagtcact aagggttagt tagttagatt agcagaaagt 4921 caaaagcctc cgaccggagg cttttgacta aaacttccct tggggttatc attggggctc 4981 actcaaaggc ggtaatcaga taaaaaaaat ccttagcttt cgctaaggat gatttctgct 5041 agagatggaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc 5101 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg atctcgcggt 5161 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg 5221 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg 5281 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa 5341 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa 5401 atcccttaac gtgagttttc gttccactga gcgtcagacc ccttaataag atgatcttct 5461 tgagatcgtt ttggtctgcg cgtaatctct tgctctgaaa acgaaaaaac cgccttgcag 5521 ggcggttttt cgaaggttct ctgagctacc aactctttga accgaggtaa ctggcttgga 5581 ggagcgcagt caccaaaact tgtcctttca gtttagcctt aaccggcgca tgacttcaag 5641 actaactcct ctaaatcaat taccagtggc tgctgccagt ggtgcttttg catgtctttc 5701 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcggact gaacgggggg 5761 ttcgtgcata cagtccagct tggagcgaac tgcctacccg gaactgagtg tcaggcgtgg 5821 aatgagacaa acgcggccat aacagcggaa tgacaccggt aaaccgaaag gcaggaacag 5881 gagagcgcac gagggagccg ccaggggaaa cgcctggtat ctttatagtc ctgtcgggtt 5941 tcgccaccac tgatttgagc gtcagatttc gtgatgcttg tcaggggggc ggagcctatg 6001 gaaaaacggc tttgccgcgg ccctctcact tccctgttaa gtatcttcct ggcatcttcc 6061 aggaaatctc cgccccgttc gtaagccatt tccgctcgcc gcagtcgaac gaccgagcgt 6121 agcgagtcag tgagcgagga agcggaatat atcctgtatc acatattctg ctgacgcacc 6181 ggtgcagcct tttttctcct gccacatgaa gcacttcact gacaccctca tcagtgccaa 6241 catagtaagc cagtatacac tccgctagcg ctgaggtctg cctcgtgaag aaggtgttgc 6301 tgactcatac caggcctgaa tcgccccatc atccagccag aaagtgaggg agccacggtt 6361 gatgagagct ttgttgtagg tggaccagtt ggtgattttg aacttttgct ttgccacgga 6421 acggtctgcg ttgtcgggaa gatgcgtgat ctgatccttc aactcagcaa aagttcgatt 6481 tattcaacaa agccacgttg tgtctcaaaa tctctgatgt tacattgcac aagataaaaa 6541 tatatcatca tgaacaataa aactgtctgc ttacataaac agtaatacaa ggggtgtt //