IBMc120 1 pSB4A3-PECF20-B30-mScarlet-I-B15 Creator: Trevor Y. H. Ho Principal Investigator: Baojun Wang BioSafety Level: Level 1 Backbone: pSB4A3 Origin of replication: pSC101 Selection Markers: Ampicillin pSB4A3-PECF20-B30-mScarlet-I-B15 ECF20 reporter plasmid Trevor Y. H. Ho Baojun Wang 18-NOV-2020 ds-DNA annotation5 1 2ndVal-mScarlet-I #ff9ccd #ff9ccd 2ndVal-mScarlet-I range 1 1651 2349 annotation9 1 VF2 #75c6a9 #75c6a9 VF2 range 1 1422 1441 annotation7 1 RFC10 Suffix #9eafd2 #9eafd2 RFC10 Suffix range 1 2490 2510 annotation6 1 VR #b4abac #b4abac VR range 1 2644 2663 annotation0 1 repA #ff9ccd #ff9ccd repA range 1 2871 3821 annotation4 1 pSC101 origin #b7e6d7 #b7e6d7 join pSC101 origin range1 1 position0 1 441 range0 1 position1 2670 4265 annotation2 1 PECF20 #ff9ccd #ff9ccd PECF20 range 1 1562 1621 annotation3 1 BBa_B0015 #c7b0e3 #c7b0e3 BBa_B0015 range 1 2361 2489 annotation1 1 RFC10 Prefix (full) #d6b295 #d6b295 RFC10 Prefix (full) range 1 1540 1561 annotation8 1 BBa_B0030 #c7b0e3 #c7b0e3 BBa_B0030 range 1 1630 1644 IBMc120_seq 1 gtgacattcagttcgctgcgctcacggctctggcagtgaatgggggtaaatggcactacaggcgccttttatggattcatgcaaggaaactacccataatacaagaaaagcccgtcacgggcttctcagggcgttttatggcgggtctgctatgtggtgctatctgactttttgctgttcagcagttcctgccctctgattttccagtctgaccacttcggattatcccgtgacaggtcattcagactggctaatgcacccagtaaggcagcggtatcatcaacaggcttacccgtcttactgtccctagtgcttggattctcaccaataaaaaacgcccggcggcaaccgagcgttctgaacaaatccagatggagttctgaggtcattactggatctatcaacaggagtccaagcgagctcgtaaacttggtctgacagttaccaatgcttaatcagtgaggcacctatctcagcgatctgtctatttcgttcatccatagttgcctgactccccgtcgtgtagataactacgatacgggagggcttaccatctggccccagtgctgcaatgataccgcgagacccacgctcaccggctccagatttatcagcaataaaccagccagccggaagggccgagcgcagaagtggtcctgcaactttatccgcctccatccagtctattaattgttgccgggaagctagagtaagtagttcgccagttaatagtttgcgcaacgttgttgccattgctacaggcatcgtggtgtcacgctcgtcgtttggtatggcttcattcagctccggttcccaacgatcaaggcgagttacatgatcccccatgttgtgcaaaaaagcggttagctccttcggtcctccgatcgttgtcagaagtaagttggccgcagtgttatcactcatggttatggcagcactgcataattctcttactgtcatgccatccgtaagatgcttttctgtgactggtgagtactcaaccaagtcattctgagaatagtgtatgcggcgaccgagttgctcttgcccggcgtcaatacgggataataccgcgccacatagcagaactttaaaagtgctcatcattggaaaacgttcttcggggcgaaaactctcaaggatcttaccgctgttgagatccagttcgatgtaacccactcgtgcacccaactgatcttcagcatcttttactttcaccagcgtttctgggtgagcaaaaacaggaaggcaaaatgccgcaaaaaagggaataagggcgacacggaaatgttgaatactcatactcttcctttttcaatattattgaagcatttatcagggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggggttccgcgcacatttcccatggtgccacctgacgtctaagaaaccattattatcatgacattaacctataaaaataggcgtatcacgaggcagaatttcagataaaaaaaatccttagctttcgctaaggatgatttctggaattcgcggccgcttctagagGCGCGGATAAAAATTTCATTTGCCCGCGACGGATTCCCCGCCCATCTATCGTTGAACCCATACTAGAGATTAAAGAGGAGAAATACTAGatggtgagtaaaggagaagctgtgattaaagagttcatgcgcttcaaagttcacatggagggttctatgaacggtcacgagttcgagatcgaaggcgaaggcgagggccgtccgtatgaaggcacccagaccgccaaactgaaagtgactaaaggcggcccgctgcctttttcctgggacatcctgagcccgcaatttatgtacggttctagggcgttcatcaaacacccagcggatatcccggactattataagcagtcttttccggaaggtttcaagtgggaacgcgtaatgaattttgaagatggtggtgccgtgaccgtcactcaggacacctccctggaggatggcaccctgatctataaagttaaactgcgtggtactaattttccacctgatggcccggtgatgcagaaaaagacgatgggttgggaggcgtctaccgaacgcttgtatccggaagatggtgtgctgaaaggcgacattaaaatggccctgcgcctgaaagatggcggccgctatctggctgacttcaaaaccacgtacaaagccaagaaacctgtgcagatgcctggcgcgtacaatgtggaccgcaaactggacatcacctctcataatgaagattatacggtggtagagcaatatgagcgctccgagggtcgtcattctaccggtggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagtagcggccgctgcagtccggcaaaaaagggcaaggtgtcaccaccctgccctttttctttaaaaccgaaaagattacttcgcgttatgcaggcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatctcgaggttacattgtcgatctgttcatggtgaacagctttaaatgcaccaaaaactcgtaaaagctctgatgtatctatcttttttacaccgttttcatctgtgcatatggacagttttccctttgatatctaacggtgaacagttgttctacttttgtttgttagtcttgatgcttcactgatagatacaagagccataagaacctcagatccttccgtatttagccagtatgttctctagtgtggttcgttgtttttgcgtgagccatgagaacgaaccattgagatcatgcttactttgcatgtcactcaaaaattttgcctcaaaactggtgagctgaatttttgcagttaaagcatcgtgtagtgtttttcttagtccgttacgtaggtaggaatctgatgtaatggttgttggtattttgtcaccattcatttttatctggttgttctcaagttcggttacgagatccatttgtctatctagttcaacttggaaaatcaacgtatcagtcgggcggcctcgcttatcaaccaccaatttcatattgctgtaagtgtttaaatctttacttattggtttcaaaacccattggttaagccttttaaactcatggtagttattttcaagcattaacatgaacttaaattcatcaaggctaatctctatatttgccttgtgagttttcttttgtgttagttcttttaataaccactcataaatcctcatagagtatttgttttcaaaagacttaacatgttccagattatattttatgaatttttttaactggaaaagataaggcaatatctcttcactaaaaactaattctaatttttcgcttgagaacttggcatagtttgtccactggaaaatctcaaagcctttaaccaaaggattcctgatttccacagttctcgtcatcagctctctggttgctttagctaatacaccataagcattttccctactgatgttcatcatctgagcgtattggttataagtgaacgataccgtccgttctttccttgtagggttttcaatcgtggggttgagtagtgccacacagcataaaattagcttggtttcatgctccgttaagtcatagcgactaatcgctagttcatttgctttgaaaacaactaattcagacatacatctcaattggtctaggtgattttaatcactataccaattgagatgggctagtcaatgataattacatgtccttttcctttgagttgtgggtatctgtaaattctgctagacctttgctggaaaacttgtaaattctgctagaccctctgtaaattccgctagacctttgtgtgttttttttgtttatattcaagtggttataatttatagaataaagaaagaataaaaaaagataaaaagaatagatcccagccctgtgtataactcactactttagtcagttccgcagtattacaaaaggatgtcgcaaacgctgtttgctcctctacaaaacagaccttaaaaccctaaaggcttaagtagcaccctcgcaagctcgggcaaatcgctgaatattccttttgtctccgaccatcaggcacctgagtcgctgtctttttc